Literature summary extracted from

Deras, M.L.; Chittur, S.V.; Davisson, V.J.
N2-hydroxyguanosine 5'-monophosphate is a time-dependent inhibitor of Escherichia coli guanosine monophosphate synthetase (1999), Biochemistry, 38, 303-310.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.3.5.2	-	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
6.3.5.2	2-fluoroinosine 5'-monophosphate	50% inhibition above 2 mM	Escherichia coli
6.3.5.2	6-Thio-GMP	-	Escherichia coli
6.3.5.2	8-aza-GMP	-	Escherichia coli
6.3.5.2	GMP	0.33 mM, 50% inhibition	Escherichia coli
6.3.5.2	N2-hydroxyguanosine 5'-monophosphate	0.0003-0.0005 mM, 50% inhibition	Escherichia coli
6.3.5.2	oxanosine	-	Escherichia coli

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
6.3.5.2	Mg2+	required for activity	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.3.5.2	ATP + xanthosine 5'-phosphate + L-glutamine + H2O	Escherichia coli	-	AMP + diphosphate + GMP + L-glutamate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.5.2	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.3.5.2	recombinant GMPS	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.5.2	ATP + xanthosine 5'-phosphate + L-glutamine + H2O	-	Escherichia coli	AMP + diphosphate + GMP + L-glutamate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.3.5.2	GMPS	-	Escherichia coli
6.3.5.2	guanosine monophosphate synthetase	-	Escherichia coli

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
6.3.5.2	0.000092	-	N2-hydroxyguanosine 5'-monophosphate	competitive vs XMP	Escherichia coli
6.3.5.2	0.33	-	GMP	-	Escherichia coli
6.3.5.2	0.4	-	8-aza-GMP	-	Escherichia coli
6.3.5.2	0.74	-	oxanosine	-	Escherichia coli
6.3.5.2	3	-	6-Thio-GMP	-	Escherichia coli

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Release 2023.1 (January 2023)