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Literature summary extracted from

  • Polgar, L.
    Oligopeptidase B: a new type of serine peptidase with a unique substrate-dependent temperature sensitivity (1999), Biochemistry, 38, 15548-15555.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 overexpressed in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 benzoyl-Arg-4-nitroanilide enzyme is inhibited by high concentrations of substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.097
-
 benzoyl-Arg-beta-naphthylamide pH 8.00, 25°C Escherichia coli
3.4.21.83 0.195
-
 benzoyl-Arg-4-nitroanilide pH 7.10, 25°C Escherichia coli
3.4.21.83 0.296
-
 benzoyl-Arg-4-nitroanilide pH 8.03, 25°C Escherichia coli
3.4.21.83 0.301
-
 benzoyl-Arg-4-nitroanilide pH 8.00, 25°C Escherichia coli
3.4.21.83 1.24
-
 benzoyl-Arg-4-nitroanilide pH 8.10, 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 81860
-
 amino acid sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.83
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 benzoyl-Arg-4-nitroanilide + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 benzoyl-Arg-Et + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 benzoyl-Arg-NH2 + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 benzoyl-arginine ethyl ester + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 benzoyl-citrulline-Et + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 additional information hydrolyzes peptides with dibasic sites much faster than monobasic substrates, hydrolyzes the peptide bond at lysine and arginine residues Escherichia coli ?
-
 ?
3.4.21.83 Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O
-
 Escherichia coli Nalpha-benzoyl-L-Arg + 2-naphthylamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.83 25
-
 optimum temperature for the reactions of amide substrates slightly below Escherichia coli
3.4.21.83 39
-
 benzoyl-arginine ethyl ester Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.83 4848
-
 benzoyl-Arg-beta-naphthylamide pH 8.00, 25°C Escherichia coli
3.4.21.83 4850
-
 benzoyl-Arg-beta-naphthylamide pH 8.00, 25°C Escherichia coli
3.4.21.83 5496
-
 benzoyl-Arg-4-nitroanilide pH 7.10, 25°C Escherichia coli
3.4.21.83 5500
-
 benzoyl-Arg-4-nitroanilide pH 7.10, 25°C Escherichia coli
3.4.21.83 6850
-
 benzoyl-Arg-4-nitroanilide pH 8.00, 25°C Escherichia coli
3.4.21.83 6852
-
 benzoyl-Arg-4-nitroanilide pH 8.00, 25°C Escherichia coli
3.4.21.83 11290
-
 benzoyl-Arg-4-nitroanilide pH 8.03, 25°C Escherichia coli
3.4.21.83 11300
-
 benzoyl-Arg-4-nitroanilide pH 8.03, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.83 8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.83 6.5 9.5
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.83 0.05
-
 benzoyl-Arg-4-nitroanilide pH 8.10, 25°C Escherichia coli