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Literature summary extracted from

  • DeSantis, G.; Shang, X.; Jones, J.B.
    Toward tailoring of the S1 pocket of subtilisin B. lentus: Chemical modification of mutant enzymes as a strategy for removing specifiity limitations (1999), Biochemistry, 38, 13391-13397.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.62 S166C site-directed mutagenesis combined with chemical modification Lederbergia lentus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.68
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-a Lederbergia lentus
3.4.21.62 0.7
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-f Lederbergia lentus
3.4.21.62 0.73
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 0.74
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-g Lederbergia lentus
3.4.21.62 0.8
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
3.4.21.62 1.09
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-e Lederbergia lentus
3.4.21.62 1.17
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
3.4.21.62 1.34
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
3.4.21.62 1.5 2 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-h Lederbergia lentus
3.4.21.62 1.6
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
3.4.21.62 1.74
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
3.4.21.62 1.9
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C mutant S166C-S-a Lederbergia lentus
3.4.21.62 1.9
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-e and S166S-f Lederbergia lentus
3.4.21.62 2
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 2.26
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
3.4.21.62 2.46
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
3.4.21.62 3.4
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
3.4.21.62 4.4
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 5.2
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
3.4.21.62 5.3
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
3.4.21.62 5.5
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
3.4.21.62 7.2
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 8.2
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-h Lederbergia lentus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Lederbergia lentus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 succinyl-AAPA-p-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?
3.4.21.62 succinyl-AAPE-p-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?
3.4.21.62 succinyl-AAPF-4-nitroanilide + H2O
-
 Lederbergia lentus succinyl-AAPF + 4-nitroaniline
-
 ?
3.4.21.62 succinyl-AAPR-p-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 EC 3.4.21.14 formerly included in Lederbergia lentus
3.4.21.62 SBL
-
 Lederbergia lentus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 0.052 2.1 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
3.4.21.62 0.16
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 0.17
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
3.4.21.62 0.35
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-h Lederbergia lentus
3.4.21.62 0.43
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
3.4.21.62 0.68
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
3.4.21.62 1.06
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
3.4.21.62 1.75
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 4.8
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-f Lederbergia lentus
3.4.21.62 6.08
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
3.4.21.62 6.08
-
 succinyl-AAPR-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
3.4.21.62 6.8
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c and S166C-S-e Lederbergia lentus
3.4.21.62 9.65
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
3.4.21.62 14.5
-
 succinyl-AAPE-p-nitroanilide pH 8.6, 25°C mutant S166C-S-a Lederbergia lentus
3.4.21.62 16.9
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
3.4.21.62 17.7
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus
3.4.21.62 23.1
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-c Lederbergia lentus
3.4.21.62 25
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-b Lederbergia lentus
3.4.21.62 28.2
-
 succinyl-AAPA-p-nitroanilide pH 8.6, 25°C mutant S166C-S-f Lederbergia lentus
3.4.21.62 29
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-g Lederbergia lentus
3.4.21.62 47
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-d Lederbergia lentus
3.4.21.62 48
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C, mutant S166C-h Lederbergia lentus
3.4.21.62 50
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-a Lederbergia lentus
3.4.21.62 67
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-i Lederbergia lentus
3.4.21.62 76
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-j Lederbergia lentus
3.4.21.62 82
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C mutant S166C-S-e Lederbergia lentus
3.4.21.62 153
-
 succinyl-AAPF-p-nitroanilide pH 8.6, 25°C wild-type Lederbergia lentus