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Literature summary extracted from

  • Taoka, S.; Widjaja, L.; Banerjee, R.
    Assignment of enzymatic functions to specific regions of the PLP-dependent heme protein cystathionine beta-synthase (1999), Biochemistry, 38, 13155-13161.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.22 S-adenosyl-L-methionine activates full length enzyme, but not the truncated core, 4 S-adenosyl-L-methionine molecules per tetramer Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 additional information CBSdeltaC143, truncated catalytic core in which the C-terminal 143 amino acid residues are deleted, higher Km for the substrates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 2
-
 L-serine pH 6.8, 37°C, full length enzyme after L-serine pre-treatment Homo sapiens
4.2.1.22 5
-
 homocysteine pH 6.8, 37°C, full length enzyme after L-serine pre-treatment Homo sapiens
4.2.1.22 5.5
-
 L-serine pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment Homo sapiens
4.2.1.22 9.7
-
 homocysteine pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment Homo sapiens
4.2.1.22 14
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment Homo sapiens
4.2.1.22 15
-
 homocysteine pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment Homo sapiens
4.2.1.22 18
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment Homo sapiens
4.2.1.22 67
-
 homocysteine pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.22 CBSdeltaC143 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-Serine + homocysteine
-
 Homo sapiens Cystathionine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.22 dimer truncated catalytic core Homo sapiens
4.2.1.22 tetramer native enzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.22 19
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment Homo sapiens
4.2.1.22 21
-
 L-serine pH 6.8, 37°C, full length enzyme after L-serine pre-treatment Homo sapiens
4.2.1.22 39
-
 L-serine pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment Homo sapiens
4.2.1.22 45
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.22 heme
-
 Homo sapiens
4.2.1.22 pyridoxal 5'-phosphate
-
 Homo sapiens