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Literature summary extracted from

  • Li, T.; Guo, N.; Xia, X.; Wang, E.D.; Wang, Y.L.
    The peptide bond between E292-A293 of Escherichia coli leucyl-tRNA synthetase is essential for its activity (1999), Biochemistry, 38, 13063-13069.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expression and assembly of the 63 and 34 kDa peptides in Escherichia coli strain KL231 cannot complement the deficient temperature-sensitive strain, while expression of peptide fragments derived by cleavage of other peptide bonds leads to assembly of a functional enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 34000
-
 x * 34000, N-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 63000, C-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 97300, recombinant wild-type enzyme, SDS-PAGE Escherichia coli
6.1.1.4 63000
-
 x * 34000, N-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 63000, C-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 97300, recombinant wild-type enzyme, SDS-PAGE Escherichia coli
6.1.1.4 97300
-
 x * 34000, N-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 63000, C-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 97300, recombinant wild-type enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Escherichia coli
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Escherichia coli
-
 class I enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.1.1.4 additional information the enzyme contains proteolytic cleavage sites at the peptide bonds between T252 and F253, E292 and A293, K327 and A328, and P368 and D369 Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant wild-type enzyme from overexpression in Escherichia coli, purification of the proteolytically derived fragments from wild-type enzyme preparation Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 2.8
-
 purified recombinant enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.1.1.4 -20°C, 10 mg/ml of enzyme, 1 month, partial cleavage of the enzyme into peptides of 63 and 34 kDA occurs, loss of 30% aminoacylation activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu the peptide bond between Glu292 and Ala293 in the large connecting polypeptide CP1 is essential for activity Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information proteolytically derived 34 kDa peptide fragment has lost most of its aminoacylation activity, but retains the ATP-dihosphate exchnage activity, the enzyme also performs the ATP-diphosphate exchange reaction Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 34000, N-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 63000, C-terminal peptide after cleavage of peptide bond E292-A293, SDS-PAGE, x * 97300, recombinant wild-type enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucine translase
-
 Escherichia coli
6.1.1.4 Leucine--tRNA ligase
-
 Escherichia coli
6.1.1.4 Leucyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.4 Leucyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.4 Leucyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.4 Leucyl-tRNA synthetase
-
 Escherichia coli
6.1.1.4 LeuRS
-
 Escherichia coli
6.1.1.4 Synthetase, leucyl-transfer ribonucleate
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 7.8
-
 ATP-diphosphate exchange assay at Escherichia coli
6.1.1.4 8
-
 aminoacylation assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
 Escherichia coli