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Literature summary extracted from

  • Gaucher, J.F.; Selkti, M.; Tiraboschi, G.; Prange, T.; Roques, B.P.; Tomas, A.; Fournie-Zaluski, M.C.
    Crystal structures of alpha-mercaptoacyldipeptides in the thermolysin active site: structural parameters for a Zn monodentation or bidentation in metalloendopeptidases (1999), Biochemistry, 38, 12569-12576.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.27 TLN-inhibitor complex, 3 alpha-mercaptoacyldipeptides in the thermolysin active site crystallized, hexagonal crystals, space group P6(1)22, [(2S)-2-sulfanyl-3-phenylpropanoyl]Gly-(5-Ph)Pro, parameters a : b : 93.50 A, c : 131.30 A, [(2S)-2-sulfanyl-3-phenylpropanoyl]Phe-Tyr, a : b : 93.97 A, c : 131.79 A, [(2S,R)-2-sulfanylheptanoyl]Phe-Ala, a : b : 93.31 A, c : 131.83 A Bacillus thermoproteolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.27 CGS-28,106
-
Bacillus thermoproteolyticus
3.4.24.27 thiorphan
-
Bacillus thermoproteolyticus
3.4.24.27 [(2S)-2-sulfanyl-3-phenylpropanoyl]Gly-(5-Ph)Pro
-
Bacillus thermoproteolyticus
3.4.24.27 [(2S)-2-sulfanyl-3-phenylpropanoyl]Phe-Tyr
-
Bacillus thermoproteolyticus
3.4.24.27 [(2S,R)-2-sulfanylheptanoyl]Phe-Ala
-
Bacillus thermoproteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 0.2
-
[3H]Tyr-Gly-Gly-Phe-Leu pH 8.0, 37°C Bacillus thermoproteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.27 34600
-
-
Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus P00800
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 Tyr-Gly-Gly-Phe-Leu + H2O
-
Bacillus thermoproteolyticus ?
-
?
3.4.24.27 [3H]Tyr-Gly-Gly-Phe-Leu + H2O
-
Bacillus thermoproteolyticus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.27 EC 3.4.24.4 formerly Bacillus thermoproteolyticus
3.4.24.27 TLN
-
Bacillus thermoproteolyticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.27 0.000042
-
[(2S)-2-sulfanyl-3-phenylpropanoyl]Phe-Tyr pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.000048
-
[(2S,R)-2-sulfanylheptanoyl]Phe-Ala pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.0012
-
[(2S)-2-sulfanyl-3-phenylpropanoyl]Gly-(5-Ph)Pro pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.0016
-
thiorphan pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.0016
-
CGS-28,106 pH 8.0, 37°C Bacillus thermoproteolyticus