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Literature summary extracted from

  • Bouhss, A.; Dementin, S.; Parquet, C.; Mengin-Lecreulx, D.; Bertrand, J.A.; Le Beller, D.; Dideberg, O.; van Heijenoort, J.; Blanot, D.
    Role of the ortholog and paralog amino acid invariants in the active site of the UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) (1999), Biochemistry, 38, 12240-12247.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.9 expressed in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.9 D317A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 D35A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 E157A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 E157K reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 H183A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 H301A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 K115A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 K198A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 K198F reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 N268A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 N271A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 R302A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 R425A reduced enzymatic activity with respect to the wild-type Escherichia coli
6.3.2.9 Y194F reduced enzymatic activity with respect to the wild-type Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.9 0.012
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, N268A mutant protein Escherichia coli
6.3.2.9 0.016
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, N271A mutant protein Escherichia coli
6.3.2.9 0.03
-
 ATP pH 9.4, 37°C, E157A mutant protein Escherichia coli
6.3.2.9 0.04
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, H301A mutant protein Escherichia coli
6.3.2.9 0.044
-
 D-glutamate pH 9.4, 37°C, D317A mutant protein Escherichia coli
6.3.2.9 0.046
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, K115A mutant protein Escherichia coli
6.3.2.9 0.053
-
 D-glutamate pH 9.4, 37°C, N271A mutant protein Escherichia coli
6.3.2.9 0.053
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, R425A mutant protein Escherichia coli
6.3.2.9 0.053
-
 D-glutamate pH 9.4, 37°C, Y194F mutant protein Escherichia coli
6.3.2.9 0.056
-
 D-glutamate pH 9.4, 37°C, E157K mutant protein Escherichia coli
6.3.2.9 0.057
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, D317A mutant protein Escherichia coli
6.3.2.9 0.059
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, H183A mutant protein Escherichia coli
6.3.2.9 0.065
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, R302A mutant protein Escherichia coli
6.3.2.9 0.074
-
 D-glutamate pH 9.4, 37°C, R302A mutant protein Escherichia coli
6.3.2.9 0.084
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, D35A mutant protein Escherichia coli
6.3.2.9 0.085
-
 ATP pH 9.4, 37°C, E157K mutant protein Escherichia coli
6.3.2.9 0.095
-
 D-glutamate pH 9.4, 37°C, D35A mutant protein Escherichia coli
6.3.2.9 0.095
-
 D-glutamate pH 9.4, 37°C, H301A mutant protein Escherichia coli
6.3.2.9 0.111
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, Y194F mutant protein Escherichia coli
6.3.2.9 0.114
-
 ATP pH 9.4, 37°C, D35A mutant protein Escherichia coli
6.3.2.9 0.12
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, K198A mutant protein Escherichia coli
6.3.2.9 0.128
-
 D-glutamate pH 9.4, 37°C, K115A mutant protein Escherichia coli
6.3.2.9 0.129
-
 ATP pH 9.4, 37°C, K198F mutant protein Escherichia coli
6.3.2.9 0.13
-
 ATP pH 9.4, 37°C, H183A mutant protein Escherichia coli
6.3.2.9 0.135
-
 ATP pH 9.4, 37°C, K198A mutant protein Escherichia coli
6.3.2.9 0.14
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, K198F mutant protein Escherichia coli
6.3.2.9 0.14
-
 ATP pH 9.4, 37°C, N268A mutant protein Escherichia coli
6.3.2.9 0.144
-
 ATP pH 9.4, 37°C, Y194F mutant protein Escherichia coli
6.3.2.9 0.153
-
 ATP pH 9.4, 37°C, H301A mutant protein Escherichia coli
6.3.2.9 0.183
-
 ATP pH 9.4, 37°C, R425A mutant protein Escherichia coli
6.3.2.9 0.185
-
 D-glutamate pH 9.4, 37°C, K198A mutant protein Escherichia coli
6.3.2.9 0.216
-
 D-glutamate pH 9.4, 37°C, K198F mutant protein Escherichia coli
6.3.2.9 0.218
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, E157K mutant protein Escherichia coli
6.3.2.9 0.264
-
 D-glutamate pH 9.4, 37°C, N268A mutant protein Escherichia coli
6.3.2.9 0.304
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, E157A mutant protein Escherichia coli
6.3.2.9 0.328
-
 ATP pH 9.4, 37°C, K115A mutant protein Escherichia coli
6.3.2.9 0.806
-
 D-glutamate pH 9.4, 37°C, H183A mutant protein Escherichia coli
6.3.2.9 1.58
-
 ATP pH 9.4, 37°C, N271A mutant protein Escherichia coli
6.3.2.9 2
-
 ATP pH 9.4, 37°C, R302A mutant protein Escherichia coli
6.3.2.9 2.18
-
 ATP pH 9.4, 37°C, D317A mutant protein Escherichia coli
6.3.2.9 5.16
-
 D-glutamate pH 9.4, 37°C, R425A mutant protein Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.9 Escherichia coli
-
 strain DH5alpha
-
6.3.2.9 Escherichia coli DH5-alpha
-
 strain DH5alpha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.9 ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 r
6.3.2.9 ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
-
 Escherichia coli DH5-alpha ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.9 0.0003
-
 D-glutamate pH 9.4, 37°C, E157K mutant protein Escherichia coli
6.3.2.9 0.0004
-
 D-glutamate pH 9.4, 37°C, H183A mutant protein Escherichia coli
6.3.2.9 0.004
-
 D-glutamate pH 9.4, 37°C, K115A mutant protein Escherichia coli
6.3.2.9 0.012
-
 D-glutamate pH 9.4, 37°C, K198F mutant protein Escherichia coli
6.3.2.9 0.016
-
 D-glutamate pH 9.4, 37°C, K198A mutant protein Escherichia coli
6.3.2.9 0.022
-
 D-glutamate pH 9.4, 37°C, D317A mutant protein Escherichia coli
6.3.2.9 0.093
-
 D-glutamate pH 9.4, 37°C, Y194F mutant protein Escherichia coli
6.3.2.9 0.3
-
 D-glutamate pH 9.4, 37°C, N271A mutant protein Escherichia coli
6.3.2.9 0.53
-
 D-glutamate pH 9.4, 37°C, R425A mutant protein Escherichia coli
6.3.2.9 0.77
-
 D-glutamate pH 9.4, 37°C, H301A mutant protein Escherichia coli
6.3.2.9 3 6 D-glutamate pH 9.4, 37°C, R302A mutant protein Escherichia coli
6.3.2.9 3.13
-
 D-glutamate pH 9.4, 37°C, D35A mutant protein Escherichia coli
6.3.2.9 3.13
-
 D-glutamate pH 9.4, 37°C, R302A mutant protein Escherichia coli
6.3.2.9 3.3
-
 D-glutamate pH 9.4, 37°C, N268A mutant protein Escherichia coli
6.3.2.9 4.27
-
 D-glutamate pH 9.4, 37°C, D35A mutant protein Escherichia coli
6.3.2.9 5.12
-
 D-glutamate pH 9.4, 37°C, N268A mutant protein Escherichia coli
6.3.2.9 6.08
-
 D-glutamate pH 9.4, 37°C, H301A mutant protein Escherichia coli
6.3.2.9 6.08
-
 D-glutamate pH 9.4, 37°C, R425A mutant protein Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.9 9.4
-
-
 Escherichia coli