Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • DeSantis, G.; Berglund, P.; Stabile, M.R.; Gold, M.; Jones, J.B.
    Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 an S1'pockets of subtilisin Bacillus lentus (1998), Biochemistry, 37, 5968-5973.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.62
-
 Lederbergia lentus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.62 M222C site-directed mutagenesis combined with chemical modification Lederbergia lentus
3.4.21.62 S156C site-directed mutagenesis combined with chemical modification Lederbergia lentus
3.4.21.62 S166C site-directed mutagenesis combined with chemical modification Lederbergia lentus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.39
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-a Lederbergia lentus
3.4.21.62 0.51
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C Lederbergia lentus
3.4.21.62 0.54
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-d Lederbergia lentus
3.4.21.62 0.55
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C, wild-type and mutant M222C-S-e Lederbergia lentus
3.4.21.62 0.6
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-c and S166C-S-f Lederbergia lentus
3.4.21.62 0.61
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-f Lederbergia lentus
3.4.21.62 0.63
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-b Lederbergia lentus
3.4.21.62 0.65
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C Lederbergia lentus
3.4.21.62 0.67
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-d Lederbergia lentus
3.4.21.62 0.68
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-b and M222-S-a Lederbergia lentus
3.4.21.62 0.69
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-c Lederbergia lentus
3.4.21.62 0.7
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-e Lederbergia lentus
3.4.21.62 0.72
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-c Lederbergia lentus
3.4.21.62 0.74
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-d Lederbergia lentus
3.4.21.62 0.77
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C Lederbergia lentus
3.4.21.62 0.78
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-e Lederbergia lentus
3.4.21.62 0.79
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-a Lederbergia lentus
3.4.21.62 0.86
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-f Lederbergia lentus
3.4.21.62 0.92
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-b Lederbergia lentus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Lederbergia lentus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62 wild-type and mutant enzymes Lederbergia lentus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 succinyl-AAPF-p-nitroanilide + H2O
-
 Lederbergia lentus succinyl-AAPF + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 EC 3.4.21.14 formerly included in Lederbergia lentus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 0.017
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-a Lederbergia lentus
3.4.21.62 0.49
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-c Lederbergia lentus
3.4.21.62 0.833
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-f Lederbergia lentus
3.4.21.62 1.04
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-f Lederbergia lentus
3.4.21.62 1.64
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-e Lederbergia lentus
3.4.21.62 2.94
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-e Lederbergia lentus
3.4.21.62 3.7
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-e Lederbergia lentus
3.4.21.62 3.8
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-e Lederbergia lentus
3.4.21.62 6.08
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-f Lederbergia lentus
3.4.21.62 6.9
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-d and M222C-S-d Lederbergia lentus
3.4.21.62 7.2
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-b Lederbergia lentus
3.4.21.62 11.8
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-b Lederbergia lentus
3.4.21.62 14.2
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C Lederbergia lentus
3.4.21.62 16.3
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-f Lederbergia lentus
3.4.21.62 17.3
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C Lederbergia lentus
3.4.21.62 17.4
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-a Lederbergia lentus
3.4.21.62 18
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-c Lederbergia lentus
3.4.21.62 21.8
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-d Lederbergia lentus
3.4.21.62 23.4
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-b Lederbergia lentus
3.4.21.62 24.2
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-c Lederbergia lentus
3.4.21.62 30
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-a Lederbergia lentus
3.4.21.62 31
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S166C-S-c Lederbergia lentus
3.4.21.62 31.6
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-e Lederbergia lentus
3.4.21.62 33.3
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant M222C-S-a Lederbergia lentus
3.4.21.62 39
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C-S-f Lederbergia lentus
3.4.21.62 43
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C mutant S156C Lederbergia lentus
3.4.21.62 48
-
 succinyl-AAPF-p-nitroanilide pH 7.5, 25°C, wild-type Lederbergia lentus