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Literature summary extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Engineering of S2 site of aqualysin I; alteration of P2 specificity by excluding P2 side chain (1998), Biochemistry, 37, 17402-17407.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.111
-
Thermus aquaticus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.111 G101A catalytic efficiencies for bulky amino acid residues in P2 site such as valine and lucine drastically decreased Thermus aquaticus
3.4.21.111 G101L reduced catalytic efficiencies for any substrate Thermus aquaticus
3.4.21.111 G101V catalytic efficiency toward glycine was retained Thermus aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.111 0.73
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
3.4.21.111 0.76
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
3.4.21.111 0.91
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 1
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
3.4.21.111 1.2
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 1.3
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 1.5
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
3.4.21.111 1.6
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 1.7
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 3.4
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
3.4.21.111 4.2
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 5.3
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
3.4.21.111 36
-
N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 39
-
N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 44
-
N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 71
-
N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 730
-
N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 1200
-
N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.111 Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Ala-Ala-Phe + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Gly-Gly-Phe + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Phe-Leu-Ala-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Phe-Leu-Ala + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
?
3.4.21.111 N-succinyl-Phe-Val-Ala-4-nitroanilide + H2O
-
Thermus aquaticus N-succinyl-Phe-Val-Ala + 4-nitroaniline
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.111 0.009
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
3.4.21.111 0.014
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
3.4.21.111 0.024
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
3.4.21.111 0.091
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
3.4.21.111 0.15
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 0.17
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 0.18
-
N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 0.18
-
N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 0.22
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 0.25
-
N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
3.4.21.111 0.88
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 0.9
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
3.4.21.111 2.5
-
N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 3.3
-
N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 4.7
-
N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 6.08
-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4.21.111 8.8
-
N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 11
-
N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus