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Literature summary extracted from
- A refined kinetic analysis of plasminogen activation by recombinant bovine tissue-type plasminogen activator indicates two interconvertible activator forms (1998), Biochemistry, 37, 12631-12639.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.68 | fibrinogen | activation induced by | Homo sapiens | |
| 3.4.21.68 | fibrinogen | activation induced by | Bos taurus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.68 | heterologously expressed in the methylotrophic yeast Pichia pastoris GS115 | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.68 | epsilon-aminocaproic acid | - |
Bos taurus |  |
| 3.4.21.68 | plasminogen activator inhibitor PAI-1 | - |
Bos taurus | |
| 3.4.21.68 | plasminogen activator inhibitor PAI-2 | - |
Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.68 | 0.0001 | - |
plasminogen | pH 7.4, 37°C | Homo sapiens | |
| 3.4.21.68 | 0.0001 | - |
plasminogen | pH 7.4, 37°C | Bos taurus | |
| 3.4.21.68 | 0.005 | - |
plasminogen | pH 7.4, 37°C, absence of fibrinogen | Bos taurus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.68 | 60000 | - |
naked tPA amino acid chain | Bos taurus |
| 3.4.21.68 | 68000 | - |
natively glycosylated tPA | Homo sapiens |
| 3.4.21.68 | 80000 | - |
mass spectroscopy, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | Homo sapiens | single-chain tPA mediated activation | plasmin + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.68 | Bos taurus | - |
bovine | - |
| 3.4.21.68 | Homo sapiens | - |
human | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.68 | - |
Homo sapiens |
| 3.4.21.68 | - |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | single-chain tPA mediated activation | Homo sapiens | plasmin + ? | - |
? | |
| 3.4.21.68 | plasminogen + H2O | - |
Bos taurus | ? | - |
? | |
| 3.4.21.68 | Val-Leu-Lys-p-nitroanilide + H2O | i.e. S-2251 | Bos taurus | p-nitroaniline + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | Tissue-type plasminogen activator | - |
Homo sapiens |
| 3.4.21.68 | Tissue-type plasminogen activator | - |
Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.68 | 0.0025 | - |
plasminogen | pH 7.4, 37°C, absence of fibrinogen | Bos taurus |
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