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Literature summary extracted from

  • Eveland, S.S.; Pompliano, D.L.; Anderson, M.S.
    Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-g-glutamate ligases: identification of a ligase superfamily (1997), Biochemistry, 36, 6223-6229.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.10 expression of naturally occuring mutant allele murF2 with A288T mutation as glutathione S-transferase fusion protein Escherichia coli
6.3.2.10 expression of point mutants, expression of wild-type and naturally occuring mutant allele murF2 with A288T mutation as glutathione S-transferase fusion proteins Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.10 A288T naturally occuring murF2 allele, expressed as a fusion protein with glutathione S-transferase, is 181fold less catalytically active than the wild-type enzyme at 30°c and even more reduced at 42°C Escherichia coli
6.3.2.10 E158A site-directed mutagenesis, 4520fold reduced activity compared to the wild-type Escherichia coli
6.3.2.10 E158D site-directed mutagenesis, 246fold reduced activity compared to the wild-type Escherichia coli
6.3.2.10 E158G site-directed mutagenesis, over 451fold reduced activity compared to the wild-type Escherichia coli
6.3.2.10 H188A site-directed mutagenesis, 203fold reduced activity compared to the wild-type Escherichia coli
6.3.2.10 H188D site-directed mutagenesis, 2990fold reduced activity compared to the wild-type Escherichia coli
6.3.2.10 H188G site-directed mutagenesis, 214fold reduced activity compared to the wild-type Escherichia coli
6.3.2.10 H188N site-directed mutagenesis, 1860fold reduced activity compared to the wild-type Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala Escherichia coli
-
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala Escherichia coli part of bacterial peptidoglycan biosynthesis ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
?
6.3.2.10 additional information Escherichia coli the specific activity of the mutant enzyme is highly reduced compared to the wild-type murF ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.10 Escherichia coli P11880 gene murF
-
6.3.2.10 Escherichia coli P11880 naturally occuring mutant murF2 allele, with A288T exchange
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine ordered kinetic mechanism, E158 and H188 are critical for enzyme activity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.10 0.04
-
purified recombinant enzyme, 42°C Escherichia coli
6.3.2.10 0.04
-
purified recombinant murF2 mutant, 42°C Escherichia coli
6.3.2.10 0.062
-
purified recombinant enzyme, 30°C Escherichia coli
6.3.2.10 0.062
-
purified recombinant murF2 mutant, 30°C Escherichia coli
6.3.2.10 11.2
-
purified recombinant wild-type enzyme, 30°C Escherichia coli
6.3.2.10 13.9
-
purified recombinant wild-type enzyme, 42°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
-
Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala formation of an acyl phosphate intermediate Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala part of bacterial peptidoglycan biosynthesis Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
?
6.3.2.10 additional information the specific activity of the mutant enzyme is highly reduced compared to the wild-type murF Escherichia coli ?
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.10 D-Ala-D-Ala-adding enzyme
-
Escherichia coli
6.3.2.10 More member of ligase superfamily Escherichia coli
6.3.2.10 Synthetase, uridine diphosphoacetylmuramoylpentapeptide
-
Escherichia coli
6.3.2.10 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase
-
Escherichia coli
6.3.2.10 UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase
-
Escherichia coli
6.3.2.10 UDPacetylmuramoylpentapeptide synthetase
-
Escherichia coli
6.3.2.10 Uridine diphosphoacetylmuramoylpentapeptide synthetase
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.10 ATP
-
Escherichia coli