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Literature summary extracted from
- Analysis of tissue plasminogen activator specificity using peptidyl fluorogenic substrates (1997), Biochemistry, 36, 2123-2131.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.68 | recombinant tPA from Genentech, 80% sc-tPA, 20% tc-tPA | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.68 | 0.00022 | - |
D-FPRANSNH-i-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens |  |
| 3.4.21.68 | 0.0003 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0003 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.00037 | - |
D-FPRANSN-C2H5-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.00038 | - |
D-FPRANSN-(CH2)6 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0004 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0004 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0004 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0004 | - |
D-FPRANSNH-C6H5CH2 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0004 | - |
D-FPRANSNH-n-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0004 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.00046 | - |
D-FPR-ANSNH-CH2COOCH3 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0005 | - |
Boc-D-VPR-ANSNHC4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.00059 | - |
D-FPRANSNH-c-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0006 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0006 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0007 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.00078 | - |
D-FPR-ANSNH-C2H4OCH3 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0008 | - |
Boc-D-FLRANSNHC3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.001 | - |
D-LGRANSNH-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0011 | - |
D-FVRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0011 | - |
D-FPRANSNH-t-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0012 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0015 | - |
D-VGRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0026 | - |
Boc-D-FPRANSNH-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0027 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.0057 | - |
D-FPRANSNH-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0066 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0073 | - |
D-FVRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0074 | - |
D-FLRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.0078 | - |
D-FVRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0081 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0085 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.0088 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.01 | - |
D-FLRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.011 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.011 | - |
D-LGRANSNH-C6H11 | pH 7.4, 25°C, tc-tPA and sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.012 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.012 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.013 | - |
D-FPRANSNH-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.013 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.013 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.014 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.014 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.015 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.015 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.015 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.016 | - |
D-FVRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.016 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.017 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.017 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.019 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.02 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.022 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.023 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.025 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.025 | - |
D-FPRANSNH-C6H5CH2 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.026 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.026 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.029 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.029 | - |
D-FPRANSNH-CH2COOCH3 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.03 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.032 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.033 | - |
D-VGRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.036 | - |
L-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.04 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.041 | - |
D-LGRANSNH-C6H11 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.047 | - |
Boc-D-FPRANSNH-C6H11 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.048 | - |
D-FPRANSNH-n-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.058 | - |
D-VGRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.063 | - |
D-FPRANSNH-CH2COOCH3 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.063 | - |
D-FPRANSNH-C6H5CH2 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.065 | - |
plasminogen | pH 7.4, 37°C | Homo sapiens | |
| 3.4.21.68 | 0.066 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.069 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.071 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.074 | - |
D-FPRANSNH-i-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.077 | - |
D-FPRANSNH-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.079 | - |
D-FPRANSNH-t-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.084 | - |
D-FPRANSNH-t-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.088 | - |
D-FPRANSNH-c-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.091 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.095 | - |
D-FPRANSNH-n-C6H13 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.096 | - |
D-FPRANSNH-n-C6H13 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.096 | - |
D-FPRANSN-(CH2)6 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.098 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.099 | - |
D-FPR-ANSNH-n-C6H13 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.1 | - |
Boc-L-VGRANSNHC4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.11 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.12 | - |
D-FPRANSNH-n-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.12 | - |
D-FPRANSN-C2H5-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.14 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.14 | - |
D-FPRANSNH-C2H4OCH3 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.15 | - |
D-FPRANSNH-c-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.18 | - |
D-FPRANSNH-C2H4OCH3 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.25 | - |
D-FPRANSN-(CH2)6 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.32 | - |
D-FPRANSN-C2H5-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.33 | - |
D-FPRANSNH-i-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.36 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.39 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.46 | - |
L-VGRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.97 | - |
L-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | Homo sapiens | - |
plasmin + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.68 | Homo sapiens | - |
human | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | (p-F)-FPRANSNH-C2H5 + H2O | - |
Homo sapiens | (p-F)-FPR + ANSNH-C2H5 | - |
? | |
| 3.4.21.68 | Boc-(p-F)-FPRANSNH-C2H5 + H2O | - |
Homo sapiens | Boc-(p-F)-FPR + ANSNH-C2H5 | - |
? | |
| 3.4.21.68 | Boc-D-FLRANSNH-C3H7 + H2O | - |
Homo sapiens | Boc-D-FLR + ANSNH-C3H7 | - |
? | |
| 3.4.21.68 | Boc-D-FPRANSNH-C6H11 + H2O | - |
Homo sapiens | Boc-D-FPR + ANSNH-C6H11 | - |
? | |
| 3.4.21.68 | Boc-D-FVRANSNH-C2H5 + H2O | - |
Homo sapiens | Boc-D-FVR + ANSNH-C2H5 | - |
? | |
| 3.4.21.68 | Boc-D-LGRANSNH-C6H11 + H2O | - |
Homo sapiens | Boc-D-LGR + ANSNH-C6H11 | - |
? | |
| 3.4.21.68 | Boc-D-LPRANSNH-C3H7 + H2O | - |
Homo sapiens | Boc-D-LPR + ANSNH-C3H7 | - |
? | |
| 3.4.21.68 | Boc-D-LSRANSNH-C3H7 + H2O | - |
Homo sapiens | Boc-D-LSR + ANSNH-C3H7 | - |
? | |
| 3.4.21.68 | Boc-D-VGRANSNH-C4H9 + H2O | - |
Homo sapiens | Boc-D-VGR + ANSNH-C4H9 | - |
? | |
| 3.4.21.68 | Boc-D-VPRANSNH-C4H9 + H2O | - |
Homo sapiens | Boc-D-VPR + ANSNH-C4H9 | - |
? | |
| 3.4.21.68 | Boc-L-FPRANSNH-C2H5 + H2O | - |
Homo sapiens | Boc-L-FPR + ANSNH-C2H5 | - |
? | |
| 3.4.21.68 | Boc-L-VGRANSNH-C4H9 + H2O | - |
Homo sapiens | Boc-L-VGR + ANSNH-C4H9 | - |
? | |
| 3.4.21.68 | D-FLRANSNH-C3H7 + H2O | - |
Homo sapiens | D-FLR + ANSNH-C3H7 | - |
? | |
| 3.4.21.68 | D-FPR ANSNH-C6H11 + H2O | - |
Homo sapiens | D-FPR + ANSNH-C6H11 | - |
? | |
| 3.4.21.68 | D-FPRANSN-(CH2)6 + H2O | - |
Homo sapiens | D-FPR + ANSN-(CH2)6 | - |
? | |
| 3.4.21.68 | D-FPRANSN-C2H5 + H2O | - |
Homo sapiens | D-FPR + ANSN-C2H5 | - |
? | |
| 3.4.21.68 | D-FPRANSN-C2H5-C2H5 + H2O | - |
Homo sapiens | D-FPR + ANSN-C2H5-C2H5 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-c-C6H11 + H2O | - |
Homo sapiens | D-FPR + ANSNH-c-C6H11 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-C2H4OCH3 + H2O | - |
Homo sapiens | D-FPR + ANSNH-C2H4OCH3 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-C6H5CH2 + H2O | - |
Homo sapiens | D-FPR + ANSNH-C6H5CH2 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-CH2COOCH3 + H2O | - |
Homo sapiens | D-FPR + ANSNH-CH2COOCH3 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-i-C3H7 + H2O | - |
Homo sapiens | D-FPR + ANSNH-i-C3H7 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-n-C4H9 + H2O | - |
Homo sapiens | D-FPR + ANSNH-n-C4H9 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-n-C6H13 + H2O | - |
Homo sapiens | D-FPR + ANSN-H-n-C6H13 | - |
? | |
| 3.4.21.68 | D-FPRANSNH-t-C4H9 + H2O | - |
Homo sapiens | D-FPR + ANSNH-t-C4H9 | - |
? | |
| 3.4.21.68 | D-FVRANSNH-C2H5 + H2O | - |
Homo sapiens | D-FVR + ANSNH-C2H5 | - |
? | |
| 3.4.21.68 | D-LGRANSNH-C6H11 + H2O | - |
Homo sapiens | D-LGR + ANSNH-C6H11 | - |
? | |
| 3.4.21.68 | D-LPRANSNH-C3H7 + H2O | - |
Homo sapiens | D-LPR + ANSNH-C3H7 | - |
? | |
| 3.4.21.68 | D-LSRANSNH-C3H7 + H2O | - |
Homo sapiens | D-LSR + ANSNH-C3H7 | - |
? | |
| 3.4.21.68 | D-VGRANSNH-C4H9 + H2O | - |
Homo sapiens | D-VGR + ANSNH-C4H9 | - |
? | |
| 3.4.21.68 | D-VPRANSNH-C4H9 + H2O | - |
Homo sapiens | D-VPR + ANSNH-C4H9 | - |
? | |
| 3.4.21.68 | L-FPRANSN-C2H5 + H2O | - |
Homo sapiens | L-FPR + ANSN-C2H5 | - |
? | |
| 3.4.21.68 | L-VGRANSNH-C4H9 + H2O | - |
Homo sapiens | L-VGR + ANSNH-C4H9 | - |
? | |
| 3.4.21.68 | additional information | single-chain t-PA, sct-PA can be converted to two-chain forrm, tct-PA, by activation cleavage at the arg275-Ile276 peptide bond, no hydrolysis of Boc-L-FLRANSNH-C3H7, L-FLRANSNH-C3H7, Boc-D-VSRANSNH-(i-C3H7), D-VSRANSNH-(i-C3H7), BOC-D-VLRANSN-H4H9, D-VLRANSN-H4H9, Boc-D-PGRANSNH-(i-C3H7), D-PGRANSNH-(i-C3H), Boc-D-PPRANSNH-(i-C3H7), D-PPRANSNH-(i-C3H7), Boc-D-SPRANSNH-CH2C6H5, D-SPRANSNH-CH2C6H5, Boc-L-SPRANSNH-CH2C6H5, L-SPRANSNH-CH2C6H5, Boc-L-(Obz)SPRANSNH-CH2C6H5, L-(Obz)SPRANSNH-CH2C6H5, (Z)-L-(Boc)EGRANSNH-C3H7, (Z)-L-EGRANSNH-CH7, L-EGRANSNH-C3H7, (Z)-L-(Boc)EPRANSNH-C3H7, (Z)-L-EPR-ANSNH-C3H7, and L-EPRANSNH-C3H7 | Homo sapiens | ? | - |
? | |
| 3.4.21.68 | plasminogen + H2O | - |
Homo sapiens | plasmin + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | sc-tPA | single-chain | Homo sapiens |
| 3.4.21.68 | tc-tPA | two-chain | Homo sapiens |
| 3.4.21.68 | Tissue plasminogen activator | - |
Homo sapiens |
| 3.4.21.68 | Tissue-type plasminogen activator | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.68 | 0.0084 | - |
D-LGRANSNH-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.01 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.011 | - |
D-FVRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.012 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.016 | - |
D-FPR-ANSNH-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.017 | - |
Boc-D-FPRANSNH-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.018 | - |
D-FLRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.019 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.019 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.021 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.021 | - |
D-VGRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.022 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.025 | - |
D-FVR-ANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.034 | - |
D-LGR-ANSNH-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.036 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.038 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.04 | - |
L-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.04 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.044 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.047 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.057 | - |
D-LGRANSNH-C6H11 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.059 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.061 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.065 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.08 | - |
L-VGRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.082 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.083 | - |
D-FPRANSNH-CH2COOCH3 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.084 | - |
Boc-L-VGRANSNHC4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.09 | - |
D-FPRANSN-C2H5-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.097 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.097 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.099 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.1 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.1 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.11 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.13 | - |
D-FPRANSN-(CH2)6 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.14 | - |
D-FPRANSN-C2H5-C2H5 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.15 | - |
Boc-D-FPRANSNH-C6H11 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.15 | - |
D-FPRANSN-H-c-C6H11 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.15 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.15 | - |
D-FPRANSNH-CH2COOCH3 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.16 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.17 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.18 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.19 | - |
D-VGRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.2 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.2 | - |
D-FPRANSN-H-i-C3H7 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.22 | - |
D-FPRANSN-(CH2)6 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.25 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.25 | - |
D-FPRANSN-H-t-C4H9 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.25 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.27 | - |
D-FPRANSNH-C2H4OCH3 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.29 | - |
D-FPRANSNH-i-C3H7 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.31 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.32 | - |
D-FPRANSNH-c-C6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.33 | - |
D-FPRANSNH-n-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.34 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.37 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.44 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.45 | - |
D-FPR-ANSNH-C6H5CH2 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.47 | - |
D-FPR-ANSNH-n-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.49 | - |
Boc-D-FVRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.51 | - |
D-FPR-ANSNHC6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.53 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.54 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.54 | - |
D-FPRANSNH-C6H5CH2 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.66 | - |
D-FPRANSNH-C2H4OCH3 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 0.69 | - |
D-FPRANSNH-i-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 0.7 | 1 | D-VPRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.71 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 0.94 | - |
D-FPRANSN-H-t-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 1.03 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 1.1 | - |
D-FPRANSNH-n-C6H13 | pH 7.4, 25°C, sc-tPA | Homo sapiens | |
| 3.4.21.68 | 1.2 | - |
D-FPRANSNH-C6H5CH2 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 1.4 | - |
D-FPRANSNH-n-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 1.47 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 1.6 | - |
D-FPRANSN-C2H5-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 1.7 | - |
D-FPRANSN-(CH2)6 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 1.8 | - |
D-FPRANSNH-CH2COOCH3 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 2.1 | - |
D-FPRANSNH-c-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 2.3 | - |
D-FVRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 2.4 | - |
D-FPRANSNH-C2H4OCH3 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 2.7 | - |
D-VPRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 2.8 | - |
D-LPRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 3.3 | - |
Boc-D-LGRANSNH-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 3.3 | - |
D-FPR-ANSNH-n-C6H13 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 3.6 | - |
D-FPR-ANSNH-n-C6H13 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 3.7 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 3.8 | - |
Boc-D-VPRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 3.8 | - |
D-FPR-ANSNH-t-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 4 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 4 | - |
D-LGR-ANSNH-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 4.4 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 4.7 | - |
Boc-D-FLRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 5 | - |
Boc-D-VGRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 5.3 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 5.3 | - |
D-LSRANSNH-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
Boc-(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
(p-F)-FPRANSNH-C2H5 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
Boc-D-LSRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
Boc-D-LPRANSNH-C3H7 | pH 7.4, 25°C | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
D-FPRANSNH-i-C3H7 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
D-FPRANSNH-C6H5CH2 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
D-FPRANSNH-C2H4OCH3 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
D-FPRANSN-H-t-C4H9 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 6.08 | - |
D-FPR-ANSNHC6H11 | pH 7.4, 25°C, tc-tPA | Homo sapiens | |
| 3.4.21.68 | 7.9 | - |
Boc-D-FVR-ANSNH-C2H5 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 9 | - |
D-VGRANSNH-C4H9 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 32 | - |
D-FPRANSNH-C6H11 | pH 7.4, 25°C, ratio tc/sc | Homo sapiens | |
| 3.4.21.68 | 89 | - |
D-LSRANSNHC3H7 | pH 7.4, 25°C | Homo sapiens | |
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