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Literature summary extracted from

  • Llano, E.; Pendas, A.M.; Knauper, V.; Sorsa, T.; Salo, T.; Salido, E.; Murphy, G.; Simmer, J.P.; Bartlett, J.D.; Lopez-Otin, C.
    Identification and structural and functional characterization of human enamelysin (MMP-20) (1997), Biochemistry, 36, 15101-15108.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B6 DNA and amino acid sequence determination and analysis, chromosome mapping to 11q22, expression in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B6 tissue matrix metalloprotease inhibitor TIMP-2 complete inhibition of amylogenin cleavage Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B6 Zn2+ enzyme contains a zinc-binding site in the catalytic domain with the consensus sequence HEXGHXXGXXH at positions 226-236 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B6 57000
-
 recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B6 amylogenin + H2O Homo sapiens involved in tooth formation ?
-
 ?
3.4.24.B6 proteins + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B6 Homo sapiens O60882
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B6 proteolytic modification enzyme proform contains a prodomain motif PRCGVPD, the proform is in a latent state Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B6 recombinant from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B6 proteolytic degradation of ameloblastin active site with the consensus sequence HEXGHXXGXXH at positions 226-236 Homo sapiens
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.B6 recombinant enzyme from inclusion bodies expressed in Escherichia coli Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B6 odontoblast
-
 Homo sapiens
-
3.4.24.B6 tooth
-
 Homo sapiens
-
3.4.24.B6 tooth enamel
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B6 amylogenin + H2O involved in tooth formation Homo sapiens ?
-
 ?
3.4.24.B6 amylogenin + H2O
-
 Homo sapiens 4 major peptide fragments of MWs 24 kDa, 23 kDa, 22 kDa, and 20 kDa
-
 ?
3.4.24.B6 Mca-PLA-norvaline-Dpa-AR-NH2 + H2O synthetic fluorogenic substrate Homo sapiens ?
-
 ?
3.4.24.B6 Mca-PLGL-Dpa-AR-NH2 + H2O synthetic fluorogenic substrate Homo sapiens ?
-
 ?
3.4.24.B6 proteins + H2O
-
 Homo sapiens peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B6 enamelysin
-
 Homo sapiens
3.4.24.B6 M10.019 Merops-ID Homo sapiens
3.4.24.B6 MMP-20
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B6 37
-
 assay at Homo sapiens