EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.27 | cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli | Bacillus thermoproteolyticus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.24.27 | - |
Bacillus thermoproteolyticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.27 | D170A | site-directed mutagenesis | Bacillus thermoproteolyticus |
3.4.24.27 | R203M | site-directed mutagenesis | Bacillus thermoproteolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.27 | phosphoramidon | - |
Bacillus thermoproteolyticus | |
3.4.24.27 | Retrothiorphan | - |
Bacillus thermoproteolyticus | |
3.4.24.27 | thiorphan | - |
Bacillus thermoproteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.2 | - |
leucine enkephalin | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.24 | - |
leucine enkephalin | pH 6.8, 37°C, mutant D170A | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.24 | - |
leucine enkephalin | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.27 | Ca2+ | contains 4 structural calcium ions | Bacillus thermoproteolyticus | |
3.4.24.27 | Zn2+ | zinc-metallopeptidase | Bacillus thermoproteolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.27 | 36000 | - |
Western blot | Bacillus thermoproteolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.27 | Bacillus thermoproteolyticus | - |
Rokko | - |
3.4.24.27 | Bacillus thermoproteolyticus Rokko | - |
Rokko | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.27 | wild-type and mutated thermolysin | Bacillus thermoproteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.27 | leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
3.4.24.27 | leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus Rokko | ? | - |
? | |
3.4.24.27 | [3H]leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
3.4.24.27 | [3H]leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus Rokko | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.083 | - |
leucine enkephalin | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus | |
3.4.24.27 | 2.94 | - |
leucine enkephalin | pH 6.8, 37°C, mutant D170A | Bacillus thermoproteolyticus | |
3.4.24.27 | 1555 | - |
leucine enkephalin | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 1560 | - |
leucine enkephalin | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.00006 | - |
phosphoramidon | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0008 | - |
Retrothiorphan | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0016 | - |
thiorphan | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0033 | - |
thiorphan | pH 6.8, 37°C, mutant D170A | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0047 | - |
phosphoramidon | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.16 | - |
thiorphan | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.16 | - |
Retrothiorphan | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus |