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Literature summary extracted from

  • Othman, A.; Lazarus, C.; Fraser, T.; Stobart, K.
    Cloning of a palmitoyl-acyl carrier protein thioesterase from oil palm (2000), Biochem. Soc. Trans., 28, 619-622.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 single gene, partial DNA sequence determination and DNA sequence analysis, expression in Escherichia coli BL21 (DE3) as glutathione S-transferase fusion protein Elaeis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.14 additional information the gene contains a putative transit peptide sequence Elaeis sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.14 66000
-
 x * 66000, recombinant glutathione S-transferase fusion protein, SDS-PAGE Elaeis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O Elaeis sp.
-
 oleic acid + acyl-carrier protein
-
 ?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O Elaeis sp. enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth palmitic acid + acyl-carrier protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Elaeis sp.
-
 oil palm
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.14 flower
-
 Elaeis sp.
-
3.1.2.14 leaf high level expression Elaeis sp.
-
3.1.2.14 mesocarp 15-18 weeks old tissue, high level expression, 8-12 weeks old tissue, low level expression Elaeis sp.
-
3.1.2.14 additional information no expression in kernel tissues Elaeis sp.
-
3.1.2.14 seedling germinating, low level expression Elaeis sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O low activity Elaeis sp. lauric acid + acyl-carrier protein
-
 ?
3.1.2.14 additional information the recombinant enzyme hydrolyzes medium- and long-chain acyl-CoAs with a 10fold lower activity compared to acyl-[acyl-carrier-protein]s Elaeis sp. ?
-
 ?
3.1.2.14 myristoyl-[acyl-carrier protein] + H2O
-
 Elaeis sp. myristic acid + acyl-carrier protein
-
 ?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O
-
 Elaeis sp. oleic acid + acyl-carrier protein
-
 ?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O best substrate Elaeis sp. palmitic acid + acyl-carrier protein
-
 ?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth Elaeis sp. palmitic acid + acyl-carrier protein
-
 ?
3.1.2.14 stearoyl-[acyl-carrier protein] + H2O
-
 Elaeis sp. stearic acid + acyl-carrier protein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.14 ? x * 66000, recombinant glutathione S-transferase fusion protein, SDS-PAGE Elaeis sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 12:0-ACP thioesterase
-
 Elaeis sp.
3.1.2.14 12:0-acyl-carrier protein thioesterase
-
 Elaeis sp.
3.1.2.14 14:0-ACP thioesterase
-
 Elaeis sp.
3.1.2.14 14:0-acyl-carrier protein thioesterase
-
 Elaeis sp.
3.1.2.14 16:0-ACP thioesterase
-
 Elaeis sp.
3.1.2.14 16:0-acyl-carrier protein thioesterase
-
 Elaeis sp.
3.1.2.14 18:0-ACP thioesterase
-
 Elaeis sp.
3.1.2.14 18:0-acyl-carrier protein thioesterase
-
 Elaeis sp.
3.1.2.14 acyl-ACP-hydrolase
-
 Elaeis sp.
3.1.2.14 acyl-acyl carrier protein hydrolase
-
 Elaeis sp.
3.1.2.14 acyl-[acyl-carrier-protein] hydrolase
-
 Elaeis sp.
3.1.2.14 AH
-
 Elaeis sp.
3.1.2.14 BTE
-
 Elaeis sp.
3.1.2.14 C12:0 ACP TE
-
 Elaeis sp.
3.1.2.14 C18:1-ACP thioesterase
-
 Elaeis sp.
3.1.2.14 Deubiquitinating enzyme 14
-
 Elaeis sp.
3.1.2.14 hydrolase, oleoyl-[acyl carrier protein]
-
 Elaeis sp.
3.1.2.14 oleoyl-ACP thioesterase
-
 Elaeis sp.
3.1.2.14 oleoyl-acyl carrier protein thioesterase
-
 Elaeis sp.
3.1.2.14 OTE
-
 Elaeis sp.
3.1.2.14 palmitoyl-acyl carrier protein thioesterase
-
 Elaeis sp.
3.1.2.14 PATE
-
 Elaeis sp.
3.1.2.14 STE
-
 Elaeis sp.
3.1.2.14 Ubiquitin thiolesterase 14
-
 Elaeis sp.
3.1.2.14 Ubiquitin-specific processing protease 14
-
 Elaeis sp.