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Literature summary extracted from

  • Cappiello, M.; Lazzarotti, A.; Buono, F.; Scaloni, A.; D'ambrosio, C.; Amodeo, P.; Mendez, B.L.; Pelosi, P.; Del Corso, A.; Mura, U.
    New role for leucyl aminopeptidase in glutathione turnover (2004), Biochem. J., 378, 35-44.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.11.1 Mn2+ is absolutely required for stability during purification Bos taurus
3.4.11.1 Mn2+ is essential for enzyme stability, in absence of Mn2+ the enzyme is inactivated within 48 h Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.42
-
 Cys-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 0.57
-
 Cys-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 0.59
-
 Cys-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 1.3
-
 L-Met-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 1.5
-
 Met-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 1.5
-
 Leu-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 2.3
-
 Leu-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 2.5
-
 Leu-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 2.8
-
 L-Met-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 5
-
 Ser-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 5.2
-
 L-Ser-Gly pH 7.4, 25°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mn2+ absolutely dependent on Bos taurus
3.4.11.1 Mn2+ dependent on Bos taurus
3.4.11.1 additional information unaffected by Zn2+ after removal of bound metal ions, while Mn2+ can restore activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 52874
-
 6 * 53700, SDS-PAGE, 6 * 52874, mass spectrometry Bos taurus
3.4.11.1 52874
-
 x * 52874, mass spectrometry Bos taurus
3.4.11.1 53700
-
 6 * 53700, SDS-PAGE, 6 * 52874, mass spectrometry Bos taurus
3.4.11.1 320000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.1 Cys-Gly + H2O Bos taurus the enzyme plays a role in glutathione turnover Cys + Gly
-
 ?
3.4.11.1 cysteinylglycine + H2O Bos taurus involved in turnover of glutathione cysteine + glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Bos taurus P00727
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 1090fold, to homogeneity Bos taurus
3.4.11.1 native enzyme 1090fold from lens to homogeneity by ion exchange chromatography, ethanol and alkaline precipitation, chloroform/octanol extraction, and gel filtration Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.1 release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low molecular modelling of enzyme-substrate binding Bos taurus
a
3.4.11.1 release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low substrate-enzyme complex structure, molecular modeling Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 lens
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 10.9
-
 purified enzyme Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.11.1 -80°C, pure enzyme, 0.2 mM MnCl2, 2 weeks, stable Bos taurus
3.4.11.1 -80°C, purified enzyme after chloroform/octanol extraction purification step, in presence of 0.2 mM Mn2+, stable for at least 2 weeks Bos taurus
3.4.11.1 4°C, partially purified enzyme after ion exchange chromatography purification step, stable for at least 6 months Bos taurus
3.4.11.1 4°C, partially purified enzyme, 6 months, stable, less stable after further purification Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Cys-Gly + H2O
-
 Bos taurus Cys + Gly
-
 ?
3.4.11.1 Cys-Gly + H2O the enzyme plays a role in glutathione turnover Bos taurus Cys + Gly
-
 ?
3.4.11.1 cysteinylglycine + H2O involved in turnover of glutathione Bos taurus cysteine + glycine
-
 ?
3.4.11.1 L-Met-Gly + H2O
-
 Bos taurus L-Met + Gly
-
 ?
3.4.11.1 L-Ser-Gly + H2O
-
 Bos taurus L-Ser + Gly
-
 ?
3.4.11.1 Leu-Gly + H2O
-
 Bos taurus Leu + Gly
-
 ?
3.4.11.1 Met-Gly + H2O
-
 Bos taurus Met + Gly
-
 ?
3.4.11.1 additional information molecular modelling of enzyme-substrate conformation, no activity with Asp-Gly Bos taurus ?
-
 ?
3.4.11.1 additional information no activity with Ser-Gly and Asp-Gly Bos taurus ?
-
 ?
3.4.11.1 Ser-Gly + H2O
-
 Bos taurus Ser + Gly
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 52874, mass spectrometry Bos taurus
3.4.11.1 hexamer 6 * 53700, SDS-PAGE, 6 * 52874, mass spectrometry Bos taurus
3.4.11.1 More primary structure analysis and molecular modeling Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 cysteinyl-glycine hydrolysing activity
-
 Bos taurus
3.4.11.1 LAP
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 25
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 2.6
-
 Cys-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 17
-
 Ser-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 42
-
 Ser-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 56.66
-
 Cys-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 57
-
 Cys-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 67
-
 Cys-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 100
-
 Cys-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 118
-
 Cys-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 118.3
-
 Cys-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 135.8
-
 Met-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 136
-
 Met-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 191.7
-
 Leu-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 192
-
 Leu-Gly pH 6.9, 25°C Bos taurus
3.4.11.1 400
-
 Leu-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 468
-
 Met-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 486.3
-
 Met-Gly pH 7.4, 25°C Bos taurus
3.4.11.1 675
-
 Leu-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 988
-
 Met-Gly pH 8.3, 25°C Bos taurus
3.4.11.1 988.3
-
 Met-Gly pH 8.3, 25°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 additional information
-
 modeling of pH-dependency Bos taurus
3.4.11.1 6.9 8.3 assay at Bos taurus
3.4.11.1 7.4
-
 assay at Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 additional information
-
 pH-dependence of activity with different dipeptide substrates Bos taurus