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Literature summary extracted from

  • Li, J.; Yao, Y.N.; Liu, M.F.; Wang, E.D.
    Arginyl-tRNA synthetase with signature sequence KMSK from Bacillus stearothermophilus (2003), Biochem. J., 376, 773-779.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.19 expression in Escherichia coli of a His-tagged recombinant enzyme, the enzyme has 73% homology with respect to Bacillus subtilis ArgRS, and 22% with respect to Escherichia coli Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.19 G136K inactive enzyme Geobacillus stearothermophilus
6.1.1.19 K382A half as active as the native enzyme Geobacillus stearothermophilus
6.1.1.19 K385A inactive enzyme Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.19 0.001
-
 L-arginine ATP-diphosphate exchange reaction, pH 8, 55°C Geobacillus stearothermophilus
6.1.1.19 0.0017
-
 tRNAArg aminoacylation reaction, pH 8, 37°C Geobacillus stearothermophilus
6.1.1.19 0.0029
-
 tRNAArg aminoacylation reaction, pH 8, 55°C Geobacillus stearothermophilus
6.1.1.19 0.0057
-
 L-arginine aminoacylation reaction, pH 8, 37°C Geobacillus stearothermophilus
6.1.1.19 0.0074
-
 L-arginine ATP-diphosphate exchange reaction, pH 8, 37°C Geobacillus stearothermophilus
6.1.1.19 0.0094
-
 L-arginine aminoacylation reaction, pH 8, 55°C Geobacillus stearothermophilus
6.1.1.19 1
-
 ATP aminoacylation reaction, pH 8, 55°C Geobacillus stearothermophilus
6.1.1.19 1.29
-
 ATP ATP-diphosphate exchange reaction, pH 8, 55°C Geobacillus stearothermophilus
6.1.1.19 1.3
-
 ATP aminoacylation reaction, pH 8, 37°C Geobacillus stearothermophilus
6.1.1.19 1.31
-
 ATP ATP-diphosphate exchange reaction, pH 8, 37°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.19 62800
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19 purification of a His-tagged recombinant enzyme using Ni-nitrilotriacetic acid agarose affinity chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Geobacillus stearothermophilus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.19 monomer 1 * 62800, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.19 55
-
 activity three-fold higher than at 37°C Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.19 65
-
 stable for 10 min Geobacillus stearothermophilus