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Literature summary extracted from

  • Board, P.G.; Taylor, M.C.; Coggan, M.; Parker, M.W.; Lantum, H.B.; Anders, M.W.
    Clarification of the role of key active site residues of glutathione transferase zeta/maleylacetoacetate isomerase by a new spectrophotometric technique (2003), Biochem. J., 374, 731-737.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 C16A mutation causes a high increase in the KM-value. Mutation diminishes activity with both chlorofluoroacetic acid and maleylacetone but shows significantly elevated activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid as a substrate. This elevated activity appears to be driven by a high turnover number. The diminished activity with maleylacetone results from a low turnover number Homo sapiens
2.5.1.18 R175A mutation results in a significant lowering of the turnover number for (+)-2-bromo-3-(4-nitrophenyl)propanoic acid and maleylacetone, elevated KM-value for maleylacetone Homo sapiens
2.5.1.18 R175K mutation results in a significant lowering of the turnover number for (+)-2-bromo-3-(4-nitrophenyl)propanoic acid and maleylacetone Homo sapiens
2.5.1.18 S14A inactive mutant enzyme Homo sapiens
2.5.1.18 S15A mutant enzyme shows low isomerase activity with maleylacetone as a substrate (about 15% of wild-type) and low activity with chlorofluoroacetic acid, it shows elevated activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid as the substrate Homo sapiens
5.2.1.2 C16A the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 2.1fold decreased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 1.6fold increased compared to wild-type enzyme Homo sapiens
5.2.1.2 R175A the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 1.5fold decreased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 6.8fold decreased compared to wild-type enzyme Homo sapiens
5.2.1.2 R175K the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 1.8fold increased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 1.3fold increased compared to wild-type enzyme Homo sapiens
5.2.1.2 S14A mutant enzyme shows no activity with {(+/-)-2-bromo-3-(4-nitrophenyl)propionic acid} or 4-maleylacetoacetate Homo sapiens
5.2.1.2 S15A the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 2.3fold decreased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 11.8fold decreased compared to wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.0139
-
 GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.0146
-
 GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.0178
-
 GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.023
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.025
-
 GSH pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.027
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.029
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1d-1d Homo sapiens
2.5.1.18 0.044
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.047
-
 GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 0.064
-
 GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 0.064
-
 maleylacetone pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.068
-
 maleylacetone pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.096
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1b-1b Homo sapiens
2.5.1.18 0.117
-
 GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 0.128
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1a-1a Homo sapiens
2.5.1.18 0.134
-
 GSH pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 0.223
-
 maleylacetone pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.287
-
 GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 0.298
-
 GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.319
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.347
-
 maleylacetone pH 7.4, 37°C, glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.417
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.768
-
 maleylacetone pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
5.2.1.2 0.023
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175A Homo sapiens
5.2.1.2 0.027
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
5.2.1.2 0.029
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1d-1d Homo sapiens
5.2.1.2 0.044
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1c-1c Homo sapiens
5.2.1.2 0.068
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme C165A Homo sapiens
5.2.1.2 0.096
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1b-1b Homo sapiens
5.2.1.2 0.128
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1a-1a Homo sapiens
5.2.1.2 0.223
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
5.2.1.2 0.319
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
5.2.1.2 0.368
-
 4-Maleylacetoacetate pH 7.6, 25°C, wild-type enzyme Homo sapiens
5.2.1.2 0.417
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme C16A Homo sapiens
5.2.1.2 0.64
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme S15A Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.18 additional information Homo sapiens the enzyme plays an important role in the metabolism of tyrosine via its maleylacetoacetate isomerase activity ?
-
 ?
5.2.1.2 additional information Homo sapiens the enzyme plays an important role in the metabolism of tyrosine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens
-
-
-
5.2.1.2 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 additional information
-
 spectrophotometric assay with 2-bromo-3-(4-nitrophenyl)propionic acid as substrate Homo sapiens
5.2.1.2 additional information
-
 spectrophotometric assay with (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid + glutathione
-
 Homo sapiens 2-(glutathion-S-yl)-3-(4-nitrophenyl)propanoic acid + HBr
-
 ?
2.5.1.18 chlorofluoroacetic acid + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 dichloroacetic acid + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 maleylacetone + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 additional information the enzyme plays an important role in the metabolism of tyrosine via its maleylacetoacetate isomerase activity Homo sapiens ?
-
 ?
5.2.1.2 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid + glutathione it is proposed that the charged side chain of Arg-175 forms a salt bridge with the carboxylate of the alpha-halo acid substrates Homo sapiens 2-(glutathion-S-yl)-3-(4-nitrophenyl)propanoic acid
-
 ?
5.2.1.2 4-maleylacetoacetate
-
 Homo sapiens 4-fumarylacetoacetate
-
 ?
5.2.1.2 additional information the enzyme plays an important role in the metabolism of tyrosine Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione transferase zeta
-
 Homo sapiens
2.5.1.18 glutathione transferase Zeta 1-1 variants glutathione transferase Zeta 1a-1a, glutathione transferase Zeta 1b-1b, glutathione transferase Zeta 1c-1c, glutathione transferase Zeta 1d-1d Homo sapiens
2.5.1.18 hGSTZ1-1
-
 Homo sapiens
5.2.1.2 glutathione S-transferase Zeta 1-1
-
 Homo sapiens
5.2.1.2 GSTZ1-1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.19
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.19
-
 GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.347
-
 maleylacetone pH 7.4, 37°C, glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.55
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1d-1d Homo sapiens
2.5.1.18 0.59
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.61
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 0.61
-
 GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 0.82
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1b-1b Homo sapiens
2.5.1.18 1
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 1
-
 GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 2.55
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 2.55
-
 GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.5.1.18 13
-
 (+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1a-1a Homo sapiens
2.5.1.18 72.6
-
 maleylacetone pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 72.6
-
 GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 141
-
 maleylacetone pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 141
-
 GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 149
-
 maleylacetone pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 149
-
 GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 368
-
 maleylacetone pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 368
-
 GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
2.5.1.18 464
-
 maleylacetone pH 7.4, 37°C, glutathione transferase Zeta 1c-1c Homo sapiens
2.5.1.18 464
-
 GSH pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
5.2.1.2 0.19
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175A Homo sapiens
5.2.1.2 0.55
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1d-1d Homo sapiens
5.2.1.2 0.59
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1c-1c Homo sapiens
5.2.1.2 0.61
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
5.2.1.2 0.82
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1b-1b Homo sapiens
5.2.1.2 1
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
5.2.1.2 1.3
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1a-1a Homo sapiens
5.2.1.2 2.1
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
5.2.1.2 2.55
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme C16A Homo sapiens
5.2.1.2 6.08
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1b-1b Homo sapiens
5.2.1.2 6.08
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1c-1c Homo sapiens
5.2.1.2 6.08
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
5.2.1.2 72.6
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
5.2.1.2 141
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme C165A Homo sapiens
5.2.1.2 149
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme R175A Homo sapiens
5.2.1.2 368
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
5.2.1.2 464
-
 4-Maleylacetoacetate pH 7.6, 25°C, wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 10
-
 activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
5.2.1.2 10.5 11 reaction with (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid and glutathione Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6.5 11 pH 6.5: about 65% of maximal activity, pH 11: about 85% of maximal activity, activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens