Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kovarik, Z.; Radic, Z.; Berman, H.A.; Simeon-Rudolf, V.; Reiner, E.; Taylor, P.
    Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates (2003), Biochem. J., 373, 33-40.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 truncated at position 548 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 F295A/Y337A mutant retains hydrolytic activity Mus musculus
3.1.1.7 F295L/Y337A mutant retains hydrolytic activity Mus musculus
3.1.1.7 F297I/Y337A increased inhibition by RP-cycloheptyl methylphosphonyl thiocholine Mus musculus
3.1.1.7 Y337A/E202Q/F295A mutant retains hydrolytic activity Mus musculus
3.1.1.7 Y337A/F295A/F297A 4fold increased Km Mus musculus
3.1.1.7 Y337A/F295L/F297I mutant retains hydrolytic activity Mus musculus
3.1.1.7 Y337A/F295L/Y338A no substrate inactivation Mus musculus
3.1.1.7 Y337A/F338A mutant retains hydrolytic activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 3,3-dimethylbutyl methylphosphonyl thiocholine
-
Mus musculus
3.1.1.7 Acetylcholine substrate inhibition Mus musculus
3.1.1.7 cycloheptyl methylphosphonyl thiocholine SP and RP enantiomers Mus musculus
3.1.1.7 isopropyl methylphosphonyl thiocholine SP and RP enantiomers Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.047
-
Acetylcholine wild-type, pH 7, 22°C Mus musculus
3.1.1.7 0.069
-
Acetylcholine Y337A/F295L/F297I mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.076
-
Acetylcholine F295A/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.1
-
Acetylcholine F295L/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.1
-
Acetylcholine Y337A/E202Q/F295A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.15
-
Acetylcholine Y337A/F295L/Y338A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.15
-
Acetylcholine Y337A/F338A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.19
-
Acetylcholine Y337A/F295A/F297A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.34
-
Acetylcholine F297I/Y337A mutant, pH 7, 22°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Mus musculus P21836
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
Mus musculus choline + acetate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.7 monomer truncated enzyme Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.7 0.017
-
Acetylcholine F295A/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.7
-
Acetylcholine F295L/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 0.97
-
Acetylcholine Y337A/E202Q/F295A mutant, pH 7, 22°C Mus musculus
3.1.1.7 16.9
-
Acetylcholine F297I/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 18.3
-
Acetylcholine Y337A/F295A/F297A mutant, pH 7, 22°C Mus musculus
3.1.1.7 23.3
-
Acetylcholine F297I/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 25
-
Acetylcholine Y337A/F295L/F297I mutant, pH 7, 22°C Mus musculus
3.1.1.7 33.33
-
Acetylcholine F295A/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 65
-
Acetylcholine Y337A/F338A mutant, pH 7, 22°C Mus musculus
3.1.1.7 66.67
-
Acetylcholine F295L/Y337A mutant, pH 7, 22°C Mus musculus
3.1.1.7 250
-
Acetylcholine wild-type, pH 7, 22°C Mus musculus