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Literature summary extracted from

  • Gordon, K.; Redelinghuys, P.; Schwager, S.L.; Ehlers, M.R.; Papageorgiou, A.C.; Natesh, R.; Acharya, K.R.; Sturrock, E.D.
    Deglycosylation, processing and crystallization of human testis angiotensin-converting enzyme (2003), Biochem. J., 371, 437-442.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.15.1 expression of wild-type enzyme and underglycosylated mutant enzymes in CHO cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.15.1 vapour-diffusion hanging-drop method, wild-type and underglycosylated mutant enzymes. The truncated soluble enzyme DELTA1-36, expressed in the presence of the glucosidase-I inhibitor N-butyldeoxynojirimycin, retains the activity of the native enzyme and yields crystals belongining to the orthorhombic P2(1)2(1)2(1) space group. Cell dimensions: a = 56.47 A, b = 84.9 A, c = 144.99 A, alpha = 90°, beta = 90° and gamma = 90° Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 additional information
-
 additional information Km-values of underglycosylated mutant enzymes Homo sapiens
3.4.15.1 2.7
-
 hippuryl-His-Leu
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.15.1 membrane integral membrane protein Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.15.1 glycoprotein N-glycosylation of Asn72 or Asn109 are necessary and sufficient for the production of enzymatically active enzyme but glycosylation of Asn90 alone results in rapid intracellular degradation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.15.1 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 hippuryl-His-Leu + H2O
-
 Homo sapiens hippuric acid + His-Leu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.15.1 additional information
-
 additional information turnover numbers of underglycosylated mutant enzymes Homo sapiens
3.4.15.1 196
-
 hippuryl-His-Leu
-
 Homo sapiens