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Literature summary extracted from

  • Nes, W.D.; Zhou, W.; Dennis, A.L.; Li, H.; Jia, Z.; Keith, R.A.; Piser, T.M.; Furlong, S.T.
    Purification, characterization and catalytic properties of human sterol 8-isomerase (2002), Biochem. J., 367, 587-599.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.5 overexpression in erg2 Saccharomyces cerevisiae and in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.5 25-Azacholesterol
-
 Homo sapiens
5.3.3.5 cholesterol
-
 Homo sapiens
5.3.3.5 emopamil
-
 Homo sapiens
5.3.3.5 ketoconazole
-
 Homo sapiens
5.3.3.5 tamoxifen
-
 Homo sapiens
5.3.3.5 tridemorph
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.5 0.05
-
 Zymosterol
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.5 26700
-
 4 * 26700, MALDI-TOF mass spectrometry Homo sapiens
5.3.3.5 27000
-
 4 * 27000, SDS-PAGE Homo sapiens
5.3.3.5 107000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.5 Homo sapiens Q15125
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.5 recombinant enzyme Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.3.5 0.243
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
5.3.3.5 -20°C, crude enzyme preparation, stable for 2 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.5 26,27-dehydrozymosterol
-
 Homo sapiens 5beta-cholest-7-en-3beta-ol
-
 ?
5.3.3.5 26,27-dinorcholesta-8,24-dienol
-
 Homo sapiens 26,27-dinorcholesta-7,24-dienol
-
 ?
5.3.3.5 26-homo-cholesta-8(9),23(24) E26(26')-trienol
-
 Homo sapiens 26-homo-cholesta-7(8),23(24)E26(26')trienol
-
 ?
5.3.3.5 5alpha-cholesta-8,24-dien-3beta-ol
-
 Homo sapiens 5alpha-cholesta-7,24-dien-3beta-ol
-
 ?
5.3.3.5 cholest-8-enol
-
 Homo sapiens cholest-7-enol
-
 ?
5.3.3.5 fecosterol
-
 Homo sapiens 24-methylene-5alpha-cholest-7-en-3beta-ol
-
 ?
5.3.3.5 additional information 3-desoxyzymosterol is not an active substrate Homo sapiens ?
-
 ?
5.3.3.5 zymosterol preferred substrate for DELTA7-sterol production, the reaction equilibrium for double-bond isomerization favours the forward direction, DELTA8 to DELTA7 Homo sapiens cholesta-7,24-dienol
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.3.5 tetramer 4 * 27000, SDS-PAGE Homo sapiens
5.3.3.5 tetramer 4 * 26700, MALDI-TOF mass spectrometry Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.5 35 45
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.5 0.423
-
 Zymosterol
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.5 7.5
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.3.5 0.001
-
 tamoxifen
-
 Homo sapiens
5.3.3.5 0.001
-
 tridemorph
-
 Homo sapiens
5.3.3.5 0.002
-
 emopamil
-
 Homo sapiens
5.3.3.5 0.021
-
 25-Azacholesterol
-
 Homo sapiens
5.3.3.5 0.156
-
 ketoconazole
-
 Homo sapiens
5.3.3.5 0.62
-
 cholesterol 37°C Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.3.5 Homo sapiens
-
-
 7.7