EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.17 | expression in Escherichia coli | Nicotiana glutinosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.17 | C338A | 14% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
4.1.1.17 | C377A | 4% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
4.1.1.17 | C96A | 42% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
4.1.1.17 | K95A | 1% of wild-type L-ornithine decarboxylase activity | Nicotiana glutinosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.17 | 5,5'-dithiobis(2-nitrobenzoic acid) | 0.001 mM, approx. 80% inhibition of ODC activity after 8 min | Nicotiana glutinosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.17 | 0.56 | - |
L-ornithine | - |
Nicotiana glutinosa | |
4.1.1.17 | 1.59 | - |
L-lysine | - |
Nicotiana glutinosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.17 | 46500 | - |
2 * 46500, SDS-PAGE | Nicotiana glutinosa |
4.1.1.17 | 92000 | - |
native PAGE | Nicotiana glutinosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.17 | L-lysine | Nicotiana glutinosa | - |
1,5-diaminopentane + CO2 | - |
? | |
4.1.1.17 | L-ornithine | Nicotiana glutinosa | - |
putrescine + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.17 | Nicotiana glutinosa | Q9FPK5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.17 | recombinant ODC | Nicotiana glutinosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.17 | L-lysine | - |
Nicotiana glutinosa | 1,5-diaminopentane + CO2 | - |
? | |
4.1.1.17 | L-ornithine | - |
Nicotiana glutinosa | putrescine + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.17 | dimer | 2 * 46500, SDS-PAGE | Nicotiana glutinosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.17 | ODC | - |
Nicotiana glutinosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.17 | 40 | - |
half-life 70 min | Nicotiana glutinosa |
4.1.1.17 | 50 | - |
half-life 20 min | Nicotiana glutinosa |
4.1.1.17 | 60 | - |
half-life 5 min | Nicotiana glutinosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.17 | 0.24 | - |
L-lysine | - |
Nicotiana glutinosa | |
4.1.1.17 | 77.8 | - |
L-ornithine | - |
Nicotiana glutinosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.17 | 6.8 | - |
L-lysine decarboxylation | Nicotiana glutinosa |
4.1.1.17 | 8 | - |
L-ornithine decarboxylation | Nicotiana glutinosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.17 | 6.5 | 7.5 | L-lysine decarboxylation, approx. 40% of maximal activity at pH 7.5 | Nicotiana glutinosa |
4.1.1.17 | 7.2 | 8.7 | L-ornithine decarboxylation, approx. 35% of maximal activity at pH 7.2 | Nicotiana glutinosa |