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Literature summary extracted from

  • Walsh, S.B.; Dolden, T.A.; Moores, G.D.; Kristensen, M.; Lewis, T.; Devonshire, A.L.; Williamson, M.S.
    Identification and characterization of mutations in housefly (Musca domestica) acetylcholinesterase involved in insecticide resistance (2001), Biochem. J., 359, 175-181.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7
-
 Musca domestica

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 F327Y mutation results in resistance to insecticides, the addition of G365A, G262A, G262V and V180L mutation has an additive effect Musca domestica
3.1.1.7 G262A mutation results in resistance to insecticides, the addition of G365A, G262V, F327Y and V180L mutation has an additive effect Musca domestica
3.1.1.7 G262A/F327Y mutation results in resistance to insecticides, the addition of G365A, G262V, and V180L mutation has an additive effect Musca domestica
3.1.1.7 G262V mutation results in strong resistance to insecticides, the addition of G365A, G262A, F327Y and V180L mutation has an additive effect Musca domestica
3.1.1.7 G262V/F327Y mutation results in resistance to insecticides, the addition of G365A, G262A, G262V, F327Y and V180L mutation has an additive effect Musca domestica
3.1.1.7 G365A mutation results in resistance to insecticides, the addition of G262A, G262V, F327Y and V180L mutation has an additive effect Musca domestica
3.1.1.7 V180L mutation results in resistance to insecticides, the addition of G365A, G262A, G262V and F327Y mutation has an additive effect Musca domestica
3.1.1.7 V180L/F327Y mutation results in resistance to insecticides, the addition of G365A, G262A and G262V mutation has an additive effect Musca domestica
3.1.1.7 V180L/G262A mutation results in resistance to insecticides, the addition of G365A, G262V and F327Y mutation has an additive effect Musca domestica
3.1.1.7 V180L/G262A/F327Y mutation results in resistance to insecticides, the addition of G365A and G262V mutation has an additive effect Musca domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 azamethiphos insecticide Musca domestica
3.1.1.7 bendiocarb insecticide Musca domestica
3.1.1.7 dichlorvos insecticide Musca domestica
3.1.1.7 malaoxon insecticide Musca domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.044
-
 acetylthiocholine iodide F327Y mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.056
-
 acetylthiocholine iodide G262A/F327Y mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.075
-
 acetylthiocholine iodide V180L mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.084
-
 acetylthiocholine iodide V180L/F327Y mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.129
-
 acetylthiocholine iodide V180L/G262A/F327Y mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.15
-
 acetylthiocholine iodide wild-type enzyme, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.153
-
 acetylthiocholine iodide V180L/G262A mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.205
-
 acetylthiocholine iodide G262A mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.21
-
 acetylthiocholine iodide G365A mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 0.267
-
 acetylthiocholine iodide G262V/F327Y mutant, 25°C, pH 7.5 Musca domestica
3.1.1.7 1.8
-
 acetylthiocholine iodide G262V mutant, 25°C, pH 7.5 Musca domestica

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Musca domestica Q95P20 housefly, variants insensitive to organophosphate and carbmate insecticides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine iodide + H2O
-
 Musca domestica thiocholine iodide + acetate
-
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