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Literature summary extracted from

  • Boileau, G.; Tenenhouse, H.S.; Desgroseillers, L.; Crine, P.
    Characterization of PHEX endopeptidase catalytic activity: identification of parathyroid-hormone-related peptide107-139 as a substrate and osteocalcin, PPi and phosphate as inhibitors (2001), Biochem. J., 355, 707-713.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.B15 additional information no effect of Ca2+ alone Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B15 PHEX gene, expression of wild-type and mutants in LLC-PK1 porcine kidney cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B15 E581V site-directed mutagenesis, soluble secreted mutant secPHEXE581V, inactive Homo sapiens
3.4.24.B15 additional information construction of an active soluble secreted mutant enzyme form secPHEX, enzyme mutations are responsible for X-chromosome linked hypophosphataemia Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B15 1,10-phenanthroline complete inhibition at 1 mM Homo sapiens
3.4.24.B15 diphosphate 50% inhibition at 2.5 mM Homo sapiens
3.4.24.B15 EDTA complete inhibition at 1 mM Homo sapiens
3.4.24.B15 additional information no effect of Ca2+ alone Homo sapiens
3.4.24.B15 osteocalcin 50% inhibition at 0.0036 mM, Ca2+ reverses the effect Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B15 membrane bound Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B15 Zn2+ zinc-metalloprotease Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B15 95000
-
 x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE Homo sapiens
3.4.24.B15 100000
-
 x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE Homo sapiens
3.4.24.B15 105000
-
 x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B15 protein + H2O Homo sapiens enzyme mutations are responsible for X-chromosome linked hypophosphataemia peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B15 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B15 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B15 recombinant soluble secreted mutant enzyme forms Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B15 proteolytic degradation of proteins at the N-terminus of aspartate residues, the S1' pocket can accomodate negatively charged side chains of amino acid residues Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B15 additional information no activity with [Leu]enkephalin, alpha-endorphin, substance P, bradykinin, big-endothelin-1, endothelin-1, alpha-calcitonin, gene-related peptide alpha-CGRP, calcitonin, osteocalcin, parathyroid-hormone-related peptide 1-84, parathyroid-hormone-related peptide 1-34, and osteogenic growth peptide Homo sapiens ?
-
 ?
3.4.24.B15 parathyroid-hormone-related peptide 107-139 + H2O
-
 Homo sapiens L-threonyl-L-arginyl-L-seryl-L-alanyl-L-tryptophyl-L-leucyl-L-alpha-aspartyl-L-serylglycyl-L-valyl-L-threonylglycyl-L-serylglycyl-L-leucyl-L-alpha-glutamylglycyl-L-a-aspartyl-L-histidyl-L-leucyl-L-serine + L-alpha-aspartyl-L-threonyl-L-seryl-L-threonyl-L-threonyl-L-seryl-L-leucyl-L-alpha-glutamyl-L-leucyl-L-alpha-aspartyl-L-seryl-L-arginine + L-threonyl-L-arginyl-L-seryl-L-alanyl-L-tryptophyl-L-leucine + L-alpha-aspartyl-L-threonyl-L-seryl-L-threonyl-L-threonyl-L-seryl-L-leucyl-L-alpha-glutamyl-L-leucine + L-alpha-aspartyl-L-seryl-L-arginine product determination ?
3.4.24.B15 protein + H2O
-
 Homo sapiens peptides
-
 ?
3.4.24.B15 protein + H2O enzyme mutations are responsible for X-chromosome linked hypophosphataemia Homo sapiens peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B15 ? x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B15 M13.091 Merops-ID Homo sapiens
3.4.24.B15 PHEX
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B15 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B15 6.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.B15 5 9 activity rapidly decreases at more basic pH values Homo sapiens