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Literature summary extracted from

  • Sommer, A.; Fischer, P.; Krause, K.; Boettcher, K.; Brophy, P.M.; Walter, R.D.; Liebau, E.
    A stress-responsive glyoxalase I from the parasitic nematode Onchocerca volvulus (2001), Biochem. J., 353, 445-452.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expression in Escherichia coli Onchocerca volvulus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 dichlorophen 0.048 mM, 50% inhibition Onchocerca volvulus
4.4.1.5 formononetin 7-O-glucoside 0.074 mM, 50% inhibition Onchocerca volvulus
4.4.1.5 oxibendazol 0.11 mM, 50% inhibition Onchocerca volvulus
4.4.1.5 purpurogallin 0.015 mM, 50% inhibition Onchocerca volvulus
4.4.1.5 S-hexylglutathione 0.11 mM, 50% inhibition Onchocerca volvulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.31
-
 Phenylglyoxal
-
 Onchocerca volvulus
4.4.1.5 0.34
-
 methylglyoxal
-
 Onchocerca volvulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 24000
-
 2 * 24000, SDS-PAGE Onchocerca volvulus
4.4.1.5 45000
-
 gel filtration Onchocerca volvulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione-methylglyoxal hemithioacetal Onchocerca volvulus glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Onchocerca volvulus
-
 causative agent of onchocerciasis, GloI expression is induced by oxidative stress
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 recombinant glyoxalase I Onchocerca volvulus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 105
-
 towards phenylglyoxal Onchocerca volvulus
4.4.1.5 284
-
 towards methylglyoxal Onchocerca volvulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal
-
 Onchocerca volvulus S-D-lactoylglutathione
-
 ?
4.4.1.5 glutathione + phenylglyoxal
-
 Onchocerca volvulus S-mandeloylglutathione
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Onchocerca volvulus (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione-phenylglyoxal hemithioacetal glutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from phenylglyoxal and glutathione Onchocerca volvulus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer 2 * 24000, SDS-PAGE Onchocerca volvulus

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 GloI
-
 Onchocerca volvulus
4.4.1.5 glyoxalase I
-
 Onchocerca volvulus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 0.53
-
 Phenylglyoxal
-
 Onchocerca volvulus
4.4.1.5 11.4
-
 methylglyoxal
-
 Onchocerca volvulus
4.4.1.5 81.7
-
 Phenylglyoxal
-
 Onchocerca volvulus