Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toogood, H.S.; Smith, C.A.; Baker, E.N.; Daniel, R.M.
    Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft (2000), Biochem. J., 350, 321-328.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.62 AkP gene cloned and expressed in Escherichia coli PB5517 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.07
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.15
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 20°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.28
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.3
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.32
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 20°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.33
-
 Suc-Ala-Ala-Pro-Ala-p-nitroanilide pH 7.5, 5°C or 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.44
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 20°C, DTT Bacillus sp. (in: Bacteria)
3.4.21.62 0.58
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 20°C, DTT Bacillus sp. (in: Bacteria)
3.4.21.62 0.63
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.68
-
 Suc-Ala-Ala-Pro-Leu-p-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 0.92
-
 Suc-Ala-Ala-Pro-Ala-p-nitroanilide pH 7.5, 75°C Bacillus sp. (in: Bacteria)
3.4.21.62 1
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 75°C Bacillus sp. (in: Bacteria)
3.4.21.62 1.01
-
 Suc-Ala-Ala-Pro-Leu-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 2.25
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 75°C Bacillus sp. (in: Bacteria)
3.4.21.62 3.37
-
 Suc-Ala-Ala-Pro-Leu-p-nitroanilide pH 7.5, 75°C Bacillus sp. (in: Bacteria)
3.4.21.62 4
-
 Suc-Ala-Ala-p-nitroanilide pH 7.5, 20°C Bacillus sp. (in: Bacteria)
3.4.21.62 6.67
-
 Suc-Ala-Ala-p-nitroanilide pH 7.5, 20°C, DTT Bacillus sp. (in: Bacteria)
3.4.21.62 8.3
-
 benzyloxycarbonyl-Gly-4-nitroanilide pH 7.5, 20°C, in presence and absence of DTT Bacillus sp. (in: Bacteria)
3.4.21.62 17
-
 Suc-Ala-Ala-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.62 Ca2+ bound calcium ions play a key role in protecting against autolysis and thermal denaturation Bacillus sp. (in: Bacteria)
3.4.21.62 Cd2+ stabilizes Bacillus sp. (in: Bacteria)
3.4.21.62 Sr2+ increases thermostability, but to a significantly lower degree than Ca2+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.62 36000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus sp. (in: Bacteria)
-
 strain Ak.1
-
3.4.21.62 Bacillus sp. (in: Bacteria) Ak1
-
 strain Ak.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62 recombinant enzyme Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.62 additional information
-
 specific activity of purified protease 174 units/mg, 1 unit = DELTA A420 of 1.0 per min at 75°C with 0.2% azocasein Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 benzyloxycarbonyl-Gly-4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) benzyloxycarbonyl-Gly + 4-nitroaniline
-
 ?
3.4.21.62 additional information little or no reaction with Glu-Phe-p-nitroanilide, benzyloxycarbonyl-Phe-p-nitroanilide, Suc-Phe-p-nitroanilide, acetyl-Phe-p-nitroanilide, Phe-p-nitroanilide, Gly-p-nitroanilide and Ala-p-nitroanilide at 75°C, and Val-Leu-Lys-p-nitroanilide, benzoyl-Arg-p-nitroanilide, Suc-Ala-Ala-p-nitroanilide, benzoyl-Tyr-p-nitroanilide, benzyloxycarbonyl-Lys-Arg-p-nitroanilide, and benzyloxycarbonyl-Arg-p-nitroanilide at 40°C Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 additional information little or no reaction with Glu-Phe-p-nitroanilide, benzyloxycarbonyl-Phe-p-nitroanilide, Suc-Phe-p-nitroanilide, acetyl-Phe-p-nitroanilide, Phe-p-nitroanilide, Gly-p-nitroanilide and Ala-p-nitroanilide at 75°C, and Val-Leu-Lys-p-nitroanilide, benzoyl-Arg-p-nitroanilide, Suc-Ala-Ala-p-nitroanilide, benzoyl-Tyr-p-nitroanilide, benzyloxycarbonyl-Lys-Arg-p-nitroanilide, and benzyloxycarbonyl-Arg-p-nitroanilide at 40°C Bacillus sp. (in: Bacteria) Ak1 ?
-
 ?
3.4.21.62 N-succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 succinyl-AAPA-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 succinyl-AAPF-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-AAPF + p-nitroaniline
-
 ?
3.4.21.62 succinyl-AAPF-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) Ak1 succinyl-AAPF + p-nitroaniline
-
 ?
3.4.21.62 Succinyl-Ala-Ala 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 Succinyl-Ala-Ala 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) Ak1 ?
-
 ?
3.4.21.62 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.4.21.62 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) Ak1 ?
-
 ?
3.4.21.62 succinyl-Ala-Ala-Ala-Ala-Ala-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-AAAAA + p-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-AAA + p-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-Ala-Ala + p-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Ala-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-AAPA + p-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-AAPL + p-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.62 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) Ak1 succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.62 succinyl-Phe-Ala-Ala-Phe-p-nitroanilide + H2O
-
 Bacillus sp. (in: Bacteria) succinyl-Phe-Ala-Ala-Phe + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 Ak.1 protease
-
 Bacillus sp. (in: Bacteria)
3.4.21.62 thermostable subtilisin
-
 Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 93
-
 Suc-Ala-Ala-p-nitroanilide pH 7.5, 20°C, in presence and absence of DTT Bacillus sp. (in: Bacteria)
3.4.21.62 148
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 222
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 222
-
 Suc-Ala-Ala-Pro-Ala-p-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 389
-
 Suc-Ala-Ala-Pro-Leu-p-nitroanilide pH 7.5, 5°C Bacillus sp. (in: Bacteria)
3.4.21.62 750
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 20°C, DTT Bacillus sp. (in: Bacteria)
3.4.21.62 1200
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 20°C Bacillus sp. (in: Bacteria)
3.4.21.62 1900
-
 benzyloxycarbonyl-Gly-4-nitroanilide pH 7.5, 20°C Bacillus sp. (in: Bacteria)
3.4.21.62 2500
-
 benzyloxycarbonyl-Gly-4-nitroanilide pH 7.5, 20°C, DTT Bacillus sp. (in: Bacteria)
3.4.21.62 2900
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 20°C, DTT Bacillus sp. (in: Bacteria)
3.4.21.62 3700
-
 Suc-Ala-Ala-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 8800
-
 Suc-Ala-Ala-Pro-Ala-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 10000
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 20°C Bacillus sp. (in: Bacteria)
3.4.21.62 22000
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 97000
-
 Suc-Ala-Ala-Pro-Leu-p-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 97000
-
 Suc-Ala-Ala-Ala-p-nitroanilide pH 7.5, 75°C Bacillus sp. (in: Bacteria)
3.4.21.62 130000
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C Bacillus sp. (in: Bacteria)
3.4.21.62 130000
-
 Suc-Ala-Ala-Pro-Ala-p-nitroanilide pH 7.5, 75°C Bacillus sp. (in: Bacteria)