BRENDA - Enzyme Database

Neutral sphingomyelinase activity dependent on Mg2+ and anionic phospholipids in the intraerythrocytic malaria parasite Plasmodium falciparum

Hanada, K.; Mitamura, T.; Fukasawa, M.; Magistrado, P.A.; Horii, T.; Nishijima, M.; Biochem. J. 346, 671-677 (2000)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
cardiolipin
anionic phospholipids required for activity, maximal activation at 1 mM, 21% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidic acid
anionic phospholipids required for activity, maximal activation at 1 mM, 33% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidylglycerol
anionic phospholipids required for activity, maximal activation at 1 mM, 79% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidylinositol
anionic phospholipids required for activity, maximal activation at 1 mM, 53% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidylserine
anionic phospholipids required for activity, maximal activation at 1 mM
Plasmodium falciparum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
scyphostatin
0.005 mM, 50% inhibition, complete inhibition at 0.033 mM
Plasmodium falciparum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.12
0.1
-
sphingomyelin
37C, pH 7.5
Plasmodium falciparum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.12
membrane
-
Plasmodium falciparum
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.12
Mg2+
required for activity, half-maximal activation at 1 mM
Plasmodium falciparum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
sphingomyelin + H2O
Plasmodium falciparum
-
N-acylsphingosine + choline phosphate
-
Plasmodium falciparum
?
3.1.4.12
sphingomyelin + H2O
Plasmodium falciparum Honduras 1
-
N-acylsphingosine + choline phosphate
-
Plasmodium falciparum Honduras 1
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.12
Plasmodium falciparum
-
no. 1-CDC
-
3.1.4.12
Plasmodium falciparum Honduras 1
-
no. 1-CDC
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
sphingomyelin + H2O
-
649682
Plasmodium falciparum
N-acylsphingosine + choline phosphate
-
649682
Plasmodium falciparum
?
3.1.4.12
sphingomyelin + H2O
-
649682
Plasmodium falciparum Honduras 1
N-acylsphingosine + choline phosphate
-
649682
Plasmodium falciparum Honduras 1
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7
8
-
Plasmodium falciparum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.12
5.5
9
less than 5% of maximal activity at pH 5.5 and pH 9.0, respectively
Plasmodium falciparum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
cardiolipin
anionic phospholipids required for activity, maximal activation at 1 mM, 21% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidic acid
anionic phospholipids required for activity, maximal activation at 1 mM, 33% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidylglycerol
anionic phospholipids required for activity, maximal activation at 1 mM, 79% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidylinositol
anionic phospholipids required for activity, maximal activation at 1 mM, 53% of activation with phosphatidylserine
Plasmodium falciparum
3.1.4.12
phosphatidylserine
anionic phospholipids required for activity, maximal activation at 1 mM
Plasmodium falciparum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
scyphostatin
0.005 mM, 50% inhibition, complete inhibition at 0.033 mM
Plasmodium falciparum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.12
0.1
-
sphingomyelin
37C, pH 7.5
Plasmodium falciparum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.12
membrane
-
Plasmodium falciparum
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.12
Mg2+
required for activity, half-maximal activation at 1 mM
Plasmodium falciparum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
sphingomyelin + H2O
Plasmodium falciparum
-
N-acylsphingosine + choline phosphate
-
Plasmodium falciparum
?
3.1.4.12
sphingomyelin + H2O
Plasmodium falciparum Honduras 1
-
N-acylsphingosine + choline phosphate
-
Plasmodium falciparum Honduras 1
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
sphingomyelin + H2O
-
649682
Plasmodium falciparum
N-acylsphingosine + choline phosphate
-
649682
Plasmodium falciparum
?
3.1.4.12
sphingomyelin + H2O
-
649682
Plasmodium falciparum Honduras 1
N-acylsphingosine + choline phosphate
-
649682
Plasmodium falciparum Honduras 1
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7
8
-
Plasmodium falciparum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.12
5.5
9
less than 5% of maximal activity at pH 5.5 and pH 9.0, respectively
Plasmodium falciparum