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Literature summary extracted from
- Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein (1999), Biochem. J., 341, 537-543.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 6.3.4.4 | agriculture | target of herbicides | Saccharomyces cerevisiae |
| 6.3.4.4 | medicine | target of drugs | Saccharomyces cerevisiae |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.3.4.4 | overexpressed | Saccharomyces cerevisiae |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 6.3.4.4 | purified enzyme is not stable, loses its activity within weeks | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.4 | adenylosuccinate | - |
Saccharomyces cerevisiae |  |
| 6.3.4.4 | AMP | - |
Saccharomyces cerevisiae | |
| 6.3.4.4 | ATP | - |
Saccharomyces cerevisiae | |
| 6.3.4.4 | Cl- | weak inhibitor | Saccharomyces cerevisiae | |
| 6.3.4.4 | GMP | potent inhibitor, competitive inhibition towards IMP | Saccharomyces cerevisiae | |
| 6.3.4.4 | additional information | negligible inhibition by adenine nucleotides | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.3.4.4 | 0.06 | - |
GTP | pH 8.0, 25°C, native enzyme | Saccharomyces cerevisiae | |
| 6.3.4.4 | 0.081 | - |
GTP | pH 8.0, 25°C, recombinant enzyme | Saccharomyces cerevisiae | |
| 6.3.4.4 | 0.2 | - |
IMP | pH 8.0, 25°C, recombinant enzyme | Saccharomyces cerevisiae | |
| 6.3.4.4 | 0.3 | - |
IMP | pH 8.0, 25°C, native enzyme | Saccharomyces cerevisiae | |
| 6.3.4.4 | 1 | - |
L-aspartate | pH 8.0, 25°C, native enzyme | Saccharomyces cerevisiae | |
| 6.3.4.4 | 1.65 | - |
L-aspartate | pH 8.0, 25°C, recombinant enzyme | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.4 | 47000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
| 6.3.4.4 | 48000 | - |
theoretical molecular mass | Saccharomyces cerevisiae |
| 6.3.4.4 | 77000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.4 | GTP + IMP + L-aspartate | Saccharomyces cerevisiae | key enzyme for the synthesis of adenosine de novo, first committed step in synythesis of adenosine, located at the branchpoint of AMP and GMP synthesis | GDP + phosphate + adenylosuccinate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.3.4.4 | Saccharomyces cerevisiae | - |
yeast | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.3.4.4 | - |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 6.3.4.4 | -20°C, remains stable for months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.4 | GTP + IMP + L-aspartate | - |
Saccharomyces cerevisiae | GDP + phosphate + adenylosuccinate | - |
? | |
| 6.3.4.4 | GTP + IMP + L-aspartate | key enzyme for the synthesis of adenosine de novo, first committed step in synythesis of adenosine, located at the branchpoint of AMP and GMP synthesis | Saccharomyces cerevisiae | GDP + phosphate + adenylosuccinate | - |
? | |
| 6.3.4.4 | additional information | ATP is no substrate | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.3.4.4 | dimer | 2 * 47000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.3.4.4 | adenosylsuccinate synthase | - |
Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.4 | 8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.4 | 6 | 10 | - |
Saccharomyces cerevisiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.3.4.4 | 0.057 | - |
GMP | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 6.3.4.4 | 2.7 | - |
adenylosuccinate | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 6.3.4.4 | 4 | - |
ATP | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 6.3.4.4 | 6 | - |
AMP | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 6.3.4.4 | 8 | - |
Cl- | pH 8.0, 25°C | Saccharomyces cerevisiae | |
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