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Literature summary extracted from

  • Binieda, A.; Fuhrmann, M.; Lehner, B.; Rey-Berthod, C.; Frutiger-Hughes, S.; Hughes, G.; Shaw, N.M.
    Purification, characterization, DNA sequence and cloning of a pimeloyl-CoA synthetase from Pseudomonas mendocina 35 (1999), Biochem. J., 340, 793-801.
No PubMed abstract available

Application

EC Number Application Comment Organism
6.2.1.14 synthesis production of biotin Pseudomonas mendocina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.14 overexpression in Escherichia coli Pseudomonas mendocina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.14 additional information
-
 additional information Michaelis-Menten kinetic Pseudomonas mendocina
6.2.1.14 0.18
-
 CoA pH 8.0, 30ºC Pseudomonas mendocina
6.2.1.14 0.49
-
 heptanedioate pH 8.0, 30ºC Pseudomonas mendocina
6.2.1.14 0.72
-
 ATP pH 8.0, 30ºC Pseudomonas mendocina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.14 soluble
-
 Pseudomonas mendocina
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.14 74643
-
 4 * 74643, calculation from sequence of cDNA Pseudomonas mendocina
6.2.1.14 298600
-
 calculated from sequence of cDNA Pseudomonas mendocina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.14 ATP + heptanedioate + CoA Pseudomonas mendocina first enzyme of biotin biosynthesis pathway AMP + diphosphate + heptanedioyl-CoA
-
 ?
6.2.1.14 ATP + heptanedioate + CoA Pseudomonas mendocina 35 first enzyme of biotin biosynthesis pathway AMP + diphosphate + heptanedioyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.14 Pseudomonas mendocina Q9ZER2
-
-
6.2.1.14 Pseudomonas mendocina 35 Q9ZER2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.14 anion exchange, gel filtration, chromatofocusing Pseudomonas mendocina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.14 cell culture
-
 Pseudomonas mendocina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.14 77.3
-
 freshly prepared enzyme, pH 8.0, at 30ºC Pseudomonas mendocina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.14 ATP + heptanedioate + CoA no activity with ethanedioate and pentanedioate Pseudomonas mendocina AMP + diphosphate + heptanedioyl-CoA
-
 ?
6.2.1.14 ATP + heptanedioate + CoA first enzyme of biotin biosynthesis pathway Pseudomonas mendocina AMP + diphosphate + heptanedioyl-CoA
-
 ?
6.2.1.14 ATP + heptanedioate + CoA no activity with ethanedioate and pentanedioate Pseudomonas mendocina 35 AMP + diphosphate + heptanedioyl-CoA
-
 ?
6.2.1.14 ATP + heptanedioate + CoA first enzyme of biotin biosynthesis pathway Pseudomonas mendocina 35 AMP + diphosphate + heptanedioyl-CoA
-
 ?
6.2.1.14 ATP + hexanedioate + CoA 72% activity of that for heptanedioate Pseudomonas mendocina AMP + diphosphate + hexanedioyl-CoA
-
 ?
6.2.1.14 ATP + hexanedioate + CoA 72% activity of that for heptanedioate Pseudomonas mendocina 35 AMP + diphosphate + hexanedioyl-CoA
-
 ?
6.2.1.14 ATP + nonanedioate + CoA 18% activity of that for heptanedioate Pseudomonas mendocina AMP + diphosphate + nonanedioyl-CoA
-
 ?
6.2.1.14 ATP + nonanedioate + CoA 18% activity of that for heptanedioate Pseudomonas mendocina 35 AMP + diphosphate + nonanedioyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.14 More subunit: estimated to be between 74000 and 80000, SDS-PAGE Pseudomonas mendocina
6.2.1.14 tetramer 4 * 74643, calculation from sequence of cDNA Pseudomonas mendocina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.14 8
-
 approximately, this value should be considered as a functional pH optimum because of the complex assay system 30ºC Pseudomonas mendocina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.14 Pseudomonas mendocina pH-gradient 6.0-4.0, chromatofocusing
-
 5.3