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Literature summary extracted from
- Kinetic properties and tissular distribution of mammalian phosphomannomutase isozymes (1999), Biochem. J., 339, 201-207.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.8 | D-Glucose 1,6-bisphosphate | PMM2 requires a hexose bisphosphate for activity, as potent as mannose 1,6-bisphosphate in activation | Homo sapiens |  |
| 5.4.2.8 | D-Mannose 1,6-bisphosphate | PMM2 requires a hexose bisphosphate for activity, as potent as glucose 1,6-bisphosphate in activation, half-maximal activation in the presence of 0.01 mM and 0.1 mM at 0.0005 mM and 0.001 mM mannose 1,6-bisphosphate, respectively | Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.8 | expression in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.8 | vanadate | 0.05 mM, 50% inhibition | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.8 | 0.012 | - |
alpha-D-glucose 1-phosphate | - |
Homo sapiens | |
| 5.4.2.8 | 0.018 | - |
D-mannose 1-phosphate | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.8 | alpha-D-mannose 1-phosphate | Homo sapiens | - |
alpha-D-mannose 6-phosphate | - |
r | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | Rattus norvegicus | 20fold higher activity than with alpha-D-glucose 1-phosphate | alpha-D-mannose 6-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.8 | Homo sapiens | - |
- |
- |
| 5.4.2.8 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.8 | recombinant PMM2, polyethylene glycol, DEAE-Sepharose, Q-Sepharose | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.8 | 49 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.8 | alpha-D-glucose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 6-phosphate | - |
r | |
| 5.4.2.8 | alpha-D-glucose 1-phosphate | - |
Homo sapiens | D-glucose 6-phosphate | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | - |
Homo sapiens | alpha-D-mannose 6-phosphate | - |
r | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | 20fold higher activity than with alpha-D-glucose 1-phosphate | Homo sapiens | alpha-D-mannose 6-phosphate | - |
r | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | 20fold higher activity than with alpha-D-glucose 1-phosphate | Rattus norvegicus | alpha-D-mannose 6-phosphate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.8 | PMM2 | - |
Homo sapiens |
| 5.4.2.8 | PMM2 | - |
Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.8 | 6.5 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.8 | 7.1 | - |
80% of maximal activity | Homo sapiens |
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Release 2023.1 (January 2023)
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