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Literature summary extracted from
- The role of the C-terminal region in phosphoglycerate mutase (1999), Biochem. J., 337, 89-95.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.11 | 2,3-bisphosphoglycerate | - |
Saccharomyces cerevisiae |  |
| 5.4.2.11 | 2-phosphoglycollate | 18-20fold activation of phosphatase activity | Saccharomyces cerevisiae | |
| 5.4.2.11 | 2-phosphoglycollate | 2.3fold activation of phosphatase activity | Schizosaccharomyces pombe | |
| 5.4.2.11 | 2-phosphohydroxypyruvate | 30fold activation of phosphatase activity | Saccharomyces cerevisiae | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.11 | - |
Schizosaccharomyces pombe |
| 5.4.2.11 | expression in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.2.11 | L245G | 39% of wild-type mutase activity, 111% of wild-type phosphatase activity | Saccharomyces cerevisiae |
| 5.4.2.11 | L245G/L246G | 105% of wild-type mutase activity, 111% of wild-type phosphatase activity | Saccharomyces cerevisiae |
| 5.4.2.11 | L246G | 98% of wild-type mutase activity, 122% of wild-type phosphatase activity | Saccharomyces cerevisiae |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.2.11 | loss of 45% of mutase activity after treatment with thermolysin | Saccharomyces cerevisiae |
| 5.4.2.11 | relatively resistant to short periods of treatment with thermolysin, both mutase and phosphatase activity decline at the same rate, approx. 40% loss of activity after 120 min treatment with thermolysin | Schizosaccharomyces pombe |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.11 | 0.22 | - |
3-phospho-D-glycerate | phosphatase activity | Saccharomyces cerevisiae | |
| 5.4.2.11 | 0.4 | - |
3-phospho-D-glycerate | L245G mutant | Saccharomyces cerevisiae | |
| 5.4.2.11 | 0.47 | - |
3-phospho-D-glycerate | mutase activity | Saccharomyces cerevisiae | |
| 5.4.2.11 | 0.58 | - |
3-phosphoglycerate | mutase activity | Schizosaccharomyces pombe | |
| 5.4.2.11 | 0.63 | - |
3-phospho-D-glycerate | L246G mutant | Saccharomyces cerevisiae | |
| 5.4.2.11 | 1.43 | - |
3-phospho-D-glycerate | L245G/L246G double mutant | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.11 | 27000 | - |
4 * 27000, SDS-PAGE | Saccharomyces cerevisiae |
| 5.4.2.11 | 27000 | - |
4 * 27000, SDS-PAGE | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.11 | 2-phospho-D-glycerate | Schizosaccharomyces pombe | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.11 | 2-phospho-D-glycerate + 2,3-bisphosphoglycerate | Saccharomyces cerevisiae | - |
3-phospho-D-glycerate + 2,3-bisphosphoglycerate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.11 | Saccharomyces cerevisiae | - |
- |
- |
| 5.4.2.11 | Schizosaccharomyces pombe | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.11 | recombinant PGAM | Schizosaccharomyces pombe |
| 5.4.2.11 | recpmbinant PGAM | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.11 | 2-phospho-D-glycerate | - |
Schizosaccharomyces pombe | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.11 | 2-phospho-D-glycerate + 2,3-bisphosphoglycerate | - |
Saccharomyces cerevisiae | 3-phospho-D-glycerate + 2,3-bisphosphoglycerate | - |
r | |
| 5.4.2.11 | 2-phospho-D-glycerate + 2,3-bisphosphoglycerate | phosphorylated PGAM also has low levels, i.e. 0.002% of mutase activity, of phosphatase activity | Saccharomyces cerevisiae | 3-phospho-D-glycerate + 2,3-bisphosphoglycerate | - |
r | |
| 5.4.2.11 | 3-Phosphoglycerate | - |
Schizosaccharomyces pombe | 2-Phosphoglycerate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.2.11 | tetramer | 4 * 27000, SDS-PAGE | Saccharomyces cerevisiae |
| 5.4.2.11 | tetramer | 4 * 27000, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.11 | PGAM | - |
Saccharomyces cerevisiae |
| 5.4.2.11 | PGAM | - |
Schizosaccharomyces pombe |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.11 | 0.0009 | - |
3-phospho-D-glycerate | phosphatase activity | Saccharomyces cerevisiae | |
| 5.4.2.11 | 0.02 | - |
3-phospho-D-glycerate | phosphatase activity | Schizosaccharomyces pombe | |
| 5.4.2.11 | 82 | - |
3-phospho-D-glycerate | mutase activity | Schizosaccharomyces pombe | |
| 5.4.2.11 | 530 | - |
3-phospho-D-glycerate | mutase activity | Saccharomyces cerevisiae | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.11 | 2,3-bisphosphoglycerate | required to prime the reaction in an initial phosphorylation step | Schizosaccharomyces pombe | |
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Release 2023.1 (January 2023)
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