BRENDA - Enzyme Database

The role of the C-terminal region in phosphoglycerate mutase

Walter, R.A.; Nairn, J.; Duncan, D.; Price, N.C.; Kelly, S.M.; Rigden, D.J.; Fothergill-Gilmore, L.A.; Biochem. J. 337, 89-95 (1999)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
5.4.2.11
2-phosphoglycollate
18-20fold activation of phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
2-phosphoglycollate
2.3fold activation of phosphatase activity
Schizosaccharomyces pombe
5.4.2.11
2-phosphohydroxypyruvate
30fold activation of phosphatase activity
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.11
-
Schizosaccharomyces pombe
5.4.2.11
expression in Saccharomyces cerevisiae
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.11
L245G
39% of wild-type mutase activity, 111% of wild-type phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
L245G/L246G
105% of wild-type mutase activity, 111% of wild-type phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
L246G
98% of wild-type mutase activity, 122% of wild-type phosphatase activity
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
5.4.2.11
loss of 45% of mutase activity after treatment with thermolysin
Saccharomyces cerevisiae
5.4.2.11
relatively resistant to short periods of treatment with thermolysin, both mutase and phosphatase activity decline at the same rate, approx. 40% loss of activity after 120 min treatment with thermolysin
Schizosaccharomyces pombe
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.22
-
3-phospho-D-glycerate
phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
0.4
-
3-phospho-D-glycerate
L245G mutant
Saccharomyces cerevisiae
5.4.2.11
0.47
-
3-phospho-D-glycerate
mutase activity
Saccharomyces cerevisiae
5.4.2.11
0.58
-
3-phosphoglycerate
mutase activity
Schizosaccharomyces pombe
5.4.2.11
0.63
-
3-phospho-D-glycerate
L246G mutant
Saccharomyces cerevisiae
5.4.2.11
1.43
-
3-phospho-D-glycerate
L245G/L246G double mutant
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.11
27000
-
4 * 27000, SDS-PAGE
Saccharomyces cerevisiae
5.4.2.11
27000
-
4 * 27000, SDS-PAGE
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Schizosaccharomyces pombe
-
3-phospho-D-glycerate
-
Schizosaccharomyces pombe
r
5.4.2.11
2-phospho-D-glycerate + 2,3-bisphosphoglycerate
Saccharomyces cerevisiae
-
3-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.11
Saccharomyces cerevisiae
-
-
-
5.4.2.11
Schizosaccharomyces pombe
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.11
recombinant PGAM
Schizosaccharomyces pombe
5.4.2.11
recpmbinant PGAM
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
649656
Schizosaccharomyces pombe
3-phospho-D-glycerate
-
649656
Schizosaccharomyces pombe
r
5.4.2.11
2-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
649656
Saccharomyces cerevisiae
3-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
649656
Saccharomyces cerevisiae
r
5.4.2.11
2-phospho-D-glycerate + 2,3-bisphosphoglycerate
phosphorylated PGAM also has low levels, i.e. 0.002% of mutase activity, of phosphatase activity
649656
Saccharomyces cerevisiae
3-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
649656
Saccharomyces cerevisiae
r
5.4.2.11
3-Phosphoglycerate
-
649656
Schizosaccharomyces pombe
2-Phosphoglycerate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.11
tetramer
4 * 27000, SDS-PAGE
Saccharomyces cerevisiae
5.4.2.11
tetramer
4 * 27000, SDS-PAGE
Schizosaccharomyces pombe
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.0009
-
3-phospho-D-glycerate
phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
0.02
-
3-phospho-D-glycerate
phosphatase activity
Schizosaccharomyces pombe
5.4.2.11
82
-
3-phospho-D-glycerate
mutase activity
Schizosaccharomyces pombe
5.4.2.11
530
-
3-phospho-D-glycerate
mutase activity
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
required to prime the reaction in an initial phosphorylation step
Schizosaccharomyces pombe
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
5.4.2.11
2-phosphoglycollate
18-20fold activation of phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
2-phosphoglycollate
2.3fold activation of phosphatase activity
Schizosaccharomyces pombe
5.4.2.11
2-phosphohydroxypyruvate
30fold activation of phosphatase activity
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.11
-
Schizosaccharomyces pombe
5.4.2.11
expression in Saccharomyces cerevisiae
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
required to prime the reaction in an initial phosphorylation step
Schizosaccharomyces pombe
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.11
L245G
39% of wild-type mutase activity, 111% of wild-type phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
L245G/L246G
105% of wild-type mutase activity, 111% of wild-type phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
L246G
98% of wild-type mutase activity, 122% of wild-type phosphatase activity
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
5.4.2.11
loss of 45% of mutase activity after treatment with thermolysin
Saccharomyces cerevisiae
5.4.2.11
relatively resistant to short periods of treatment with thermolysin, both mutase and phosphatase activity decline at the same rate, approx. 40% loss of activity after 120 min treatment with thermolysin
Schizosaccharomyces pombe
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.22
-
3-phospho-D-glycerate
phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
0.4
-
3-phospho-D-glycerate
L245G mutant
Saccharomyces cerevisiae
5.4.2.11
0.47
-
3-phospho-D-glycerate
mutase activity
Saccharomyces cerevisiae
5.4.2.11
0.58
-
3-phosphoglycerate
mutase activity
Schizosaccharomyces pombe
5.4.2.11
0.63
-
3-phospho-D-glycerate
L246G mutant
Saccharomyces cerevisiae
5.4.2.11
1.43
-
3-phospho-D-glycerate
L245G/L246G double mutant
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.11
27000
-
4 * 27000, SDS-PAGE
Saccharomyces cerevisiae
5.4.2.11
27000
-
4 * 27000, SDS-PAGE
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Schizosaccharomyces pombe
-
3-phospho-D-glycerate
-
Schizosaccharomyces pombe
r
5.4.2.11
2-phospho-D-glycerate + 2,3-bisphosphoglycerate
Saccharomyces cerevisiae
-
3-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
Saccharomyces cerevisiae
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.11
recombinant PGAM
Schizosaccharomyces pombe
5.4.2.11
recpmbinant PGAM
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
649656
Schizosaccharomyces pombe
3-phospho-D-glycerate
-
649656
Schizosaccharomyces pombe
r
5.4.2.11
2-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
649656
Saccharomyces cerevisiae
3-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
649656
Saccharomyces cerevisiae
r
5.4.2.11
2-phospho-D-glycerate + 2,3-bisphosphoglycerate
phosphorylated PGAM also has low levels, i.e. 0.002% of mutase activity, of phosphatase activity
649656
Saccharomyces cerevisiae
3-phospho-D-glycerate + 2,3-bisphosphoglycerate
-
649656
Saccharomyces cerevisiae
r
5.4.2.11
3-Phosphoglycerate
-
649656
Schizosaccharomyces pombe
2-Phosphoglycerate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.11
tetramer
4 * 27000, SDS-PAGE
Saccharomyces cerevisiae
5.4.2.11
tetramer
4 * 27000, SDS-PAGE
Schizosaccharomyces pombe
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.11
0.0009
-
3-phospho-D-glycerate
phosphatase activity
Saccharomyces cerevisiae
5.4.2.11
0.02
-
3-phospho-D-glycerate
phosphatase activity
Schizosaccharomyces pombe
5.4.2.11
82
-
3-phospho-D-glycerate
mutase activity
Schizosaccharomyces pombe
5.4.2.11
530
-
3-phospho-D-glycerate
mutase activity
Saccharomyces cerevisiae