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Literature summary extracted from

  • Warren, M.J.; Bolt, E.L.; Roessner, C.A.; Scott, A.I.; Spencer, J.B.; Woodcock, S.C.
    Gene dissection demonstrates that the Escherichia coli cysG gene encodes a multifunctional protein (1994), Biochem. J., 302, 837-844.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.76 expressed as recombinant protein Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.76 27990
-
 predicted gene derived molecular mass Escherichia coli
1.3.1.76 29000
-
 SDS-PAGE Escherichia coli
1.3.1.76 60000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+ Escherichia coli multifunctional protein involved in S-adenosyl-L-methionine-dependent methylation, pyridine dinucleotide dependent dehydrogenation, and ferrochelation, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ Escherichia coli CR252 multifunctional protein involved in S-adenosyl-L-methionine-dependent methylation, pyridine dinucleotide dependent dehydrogenation, and ferrochelation, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.76 Escherichia coli
-
-
-
1.3.1.76 Escherichia coli CR252
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.76
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+
-
 Escherichia coli sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ multifunctional protein involved in S-adenosyl-L-methionine-dependent methylation, pyridine dinucleotide dependent dehydrogenation, and ferrochelation, second of three steps leading to formation of siroheme from uroporphyrinogen III Escherichia coli sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+
-
 Escherichia coli CR252 sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 precorrin-2 + NAD+ multifunctional protein involved in S-adenosyl-L-methionine-dependent methylation, pyridine dinucleotide dependent dehydrogenation, and ferrochelation, second of three steps leading to formation of siroheme from uroporphyrinogen III Escherichia coli CR252 sirohydrochlorin + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.76 homodimer 2 * 29000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.3.1.76 CysG
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.76 NAD+
-
 Escherichia coli