Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zera, A.J.; Zeisset, M.
    Biochemical characterization of juvenile hormone esterases from lines selected for high or low enzyme activity in Gryllus assimilis (1996), Biochem. Genet., 34, 421-435.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.59 3-octylthio-1,1,1-trifluoro-2-propanone potent inhibitor, pI50: 7.3-7.8, high- and low-selected JHE activity lines Gryllus assimilis
3.1.1.59 eserine IC50: 3.9-4.2, high- and low-selected JHE activity lines Gryllus assimilis
3.1.1.59 O,O-diisopropyl phosphofluoridate pI50: 5-5.7, high- and low-selected JHE activity lines Gryllus assimilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.59 additional information
-
 additional information Km-values for juvenile hormone III from lines selected for high or low JHE activity Gryllus assimilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.59 juvenile hormone + H2O Gryllus assimilis endocrine regulator, degrades and inactivates a key developmental and reproductive hormone, juvenile hormone ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.59 Gryllus assimilis
-
 three pairs of high- and low-selected JHE activity lines, no multiple isozymes
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.59 hemolymph day 4-7 of the 10-day last stadium Gryllus assimilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.59 additional information
-
-
 Gryllus assimilis

Storage Stability

EC Number Storage Stability Organism
3.1.1.59 storage on ice, 0.1 M phosphate buffer, pH 7.1, up to 3 weeks, stable Gryllus assimilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.59 juvenile hormone + H2O endocrine regulator, degrades and inactivates a key developmental and reproductive hormone, juvenile hormone Gryllus assimilis ?
-
 ?
3.1.1.59 juvenile hormone III + H2O racemic Gryllus assimilis juvenile hormone III acid + methanol juvenile hormone acid is the sole product ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.59 JHE
-
 Gryllus assimilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.59 30
-
 assay at Gryllus assimilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.59 60.4 61.8 5 min, 50% loss of activity, high- and low-selected JHE activity lines Gryllus assimilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.59 7.1
-
 assay at Gryllus assimilis