Any feedback?
Literature summary extracted from
- Characterization of two different 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6 (2003), Biochem. Biophys. Res. Commun., 312, 1297-1302.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.7.3 | expression of forDABGE in Escherichia coli JM109, structure of gene cluster | Hydrogenobacter thermophilus |
| 1.2.7.3 | expression of korAB in Escherichia coli JM109, structure of gene cluster | Hydrogenobacter thermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.3 | 0.026 | - |
CoA | pH 7.8, 70°C, recombinant For | Hydrogenobacter thermophilus |  |
| 1.2.7.3 | 0.08 | - |
CoA | pH 7.8, 70°C, recombinant Kor | Hydrogenobacter thermophilus | |
| 1.2.7.3 | 1.4 | - |
2-oxoglutarate | pH 7.8, 70°C, recombinant Kor | Hydrogenobacter thermophilus | |
| 1.2.7.3 | 2.9 | - |
2-oxoglutarate | pH 7.8, 70°C, recombinant For | Hydrogenobacter thermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | 230000 | - |
recombinant For, gel filtration | Hydrogenobacter thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
| 1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.7.3 | Hydrogenobacter thermophilus | D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 | D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For | - |
| 1.2.7.3 | Hydrogenobacter thermophilus | Q9AJM0 and Q9AJL9 | Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For | - |
| 1.2.7.3 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 | D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For | - |
| 1.2.7.3 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | Q9AJM0 and Q9AJL9 | Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.7.3 | - |
Hydrogenobacter thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | additional information | - |
the specific activity of For is approximately one-tenth of that of Kor | Hydrogenobacter thermophilus |
| 1.2.7.3 | 35.2 | - |
pH 7.8, 70°C, recombinant Kor | Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
| 1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
| 1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
| 1.2.7.3 | 2-oxoglutarate + CoA + oxidized ferredoxin | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.7.3 | oligomer | the For enzyme has a five-subunit-type structure, the molar ratio of ForA:ForB:ForG:ForD:ForE is 1.0:1.04:1.02:0.98:0.2, the sum of the calculated MWs of the five For subunits is 136 kDa | Hydrogenobacter thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.7.3 | FOR enzyme | - |
Hydrogenobacter thermophilus |
| 1.2.7.3 | KOR enzyme | - |
Hydrogenobacter thermophilus |
| 1.2.7.3 | OGOR | - |
Hydrogenobacter thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | 80 | - |
recombinant For enzyme | Hydrogenobacter thermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | 60 | 90 | 60°C: about 35% of maximal activity, 90°C: about 50% of maximal activity, recombinant For, the recombinant For enzyme is inactive at room temperature | Hydrogenobacter thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | 70 | - |
recombinant Kor, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
| 1.2.7.3 | 80 | - |
recombinant For, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
| 1.2.7.3 | 80 | - |
recombinant Kor, about 95% loss of activity under aerobic conditions, about 25% loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
| 1.2.7.3 | 90 | - |
recombinant For, about 90% loss of activity under aerobic conditions, about 30% loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | 7.7 | - |
recombinant Kor | Hydrogenobacter thermophilus |
| 1.2.7.3 | 8.7 | - |
recombinant For, at 70°C, optimum varies between buffers | Hydrogenobacter thermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.3 | 7 | 9.7 | pH 7.0: about 45% of maximal activity, pH 9.7: about 65% of maximal activity, recombinant For | Hydrogenobacter thermophilus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.2.7.3 | Hydrogenobacter thermophilus | the For enzyme is expressed constitutively during oxygen respiration. Little expression of For is observed during cultivation under conditions of nitrate respiration | additional information |
| 1.2.7.3 | Hydrogenobacter thermophilus | the Kor enzyme is constitutively expressed during respiration of either oxygen or nitrate respiration | additional information |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.2.7.3 | physiological function | the For enzyme supports the optimal growth of the organism in the presence of oxygen | Hydrogenobacter thermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
