BRENDA - Enzyme Database show

Characterization of two different 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6

Yamamoto, M.; Arai, H.; Ishii, M.; Igarashi, Y.; Biochem. Biophys. Res. Commun. 312, 1297-1302 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.7.3
expression of forDABGE in Escherichia coli JM109, structure of gene cluster; expression of korAB in Escherichia coli JM109, structure of gene cluster
Hydrogenobacter thermophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.3
0.026
-
CoA
pH 7.8, 70C, recombinant For
Hydrogenobacter thermophilus
1.2.7.3
0.08
-
CoA
pH 7.8, 70C, recombinant Kor
Hydrogenobacter thermophilus
1.2.7.3
1.4
-
2-oxoglutarate
pH 7.8, 70C, recombinant Kor
Hydrogenobacter thermophilus
1.2.7.3
2.9
-
2-oxoglutarate
pH 7.8, 70C, recombinant For
Hydrogenobacter thermophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.3
230000
-
recombinant For, gel filtration
Hydrogenobacter thermophilus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.3
Hydrogenobacter thermophilus
D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0
D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For
-
1.2.7.3
Hydrogenobacter thermophilus
Q9AJM0 and Q9AJL9
Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For
-
1.2.7.3
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0
D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For
-
1.2.7.3
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q9AJM0 and Q9AJL9
Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.3
;
Hydrogenobacter thermophilus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.7.3
additional information
-
the specific activity of For is approximately one-tenth of that of Kor
Hydrogenobacter thermophilus
1.2.7.3
35.2
-
pH 7.8, 70C, recombinant Kor
Hydrogenobacter thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649630
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
649630
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649630
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
649630
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.3
oligomer
the For enzyme has a five-subunit-type structure, the molar ratio of ForA:ForB:ForG:ForD:ForE is 1.0:1.04:1.02:0.98:0.2, the sum of the calculated MWs of the five For subunits is 136 kDa
Hydrogenobacter thermophilus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.7.3
80
-
recombinant For enzyme
Hydrogenobacter thermophilus
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.2.7.3
60
90
60C: about 35% of maximal activity, 90C: about 50% of maximal activity, recombinant For, the recombinant For enzyme is inactive at room temperature
Hydrogenobacter thermophilus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.7.3
70
-
recombinant Kor, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
1.2.7.3
80
-
recombinant For, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions; recombinant Kor, about 95% loss of activity under aerobic conditions, about 25% loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
1.2.7.3
90
-
recombinant For, about 90% loss of activity under aerobic conditions, about 30% loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.3
7.7
-
recombinant Kor
Hydrogenobacter thermophilus
1.2.7.3
8.7
-
recombinant For, at 70C, optimum varies between buffers
Hydrogenobacter thermophilus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.7.3
7
9.7
pH 7.0: about 45% of maximal activity, pH 9.7: about 65% of maximal activity, recombinant For
Hydrogenobacter thermophilus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.3
expression of forDABGE in Escherichia coli JM109, structure of gene cluster
Hydrogenobacter thermophilus
1.2.7.3
expression of korAB in Escherichia coli JM109, structure of gene cluster
Hydrogenobacter thermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.3
0.026
-
CoA
pH 7.8, 70C, recombinant For
Hydrogenobacter thermophilus
1.2.7.3
0.08
-
CoA
pH 7.8, 70C, recombinant Kor
Hydrogenobacter thermophilus
1.2.7.3
1.4
-
2-oxoglutarate
pH 7.8, 70C, recombinant Kor
Hydrogenobacter thermophilus
1.2.7.3
2.9
-
2-oxoglutarate
pH 7.8, 70C, recombinant For
Hydrogenobacter thermophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.3
230000
-
recombinant For, gel filtration
Hydrogenobacter thermophilus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.3
-
Hydrogenobacter thermophilus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.7.3
additional information
-
the specific activity of For is approximately one-tenth of that of Kor
Hydrogenobacter thermophilus
1.2.7.3
35.2
-
pH 7.8, 70C, recombinant Kor
Hydrogenobacter thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649630
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
649630
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649630
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
649630
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.3
oligomer
the For enzyme has a five-subunit-type structure, the molar ratio of ForA:ForB:ForG:ForD:ForE is 1.0:1.04:1.02:0.98:0.2, the sum of the calculated MWs of the five For subunits is 136 kDa
Hydrogenobacter thermophilus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.7.3
80
-
recombinant For enzyme
Hydrogenobacter thermophilus
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.2.7.3
60
90
60C: about 35% of maximal activity, 90C: about 50% of maximal activity, recombinant For, the recombinant For enzyme is inactive at room temperature
Hydrogenobacter thermophilus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.7.3
70
-
recombinant Kor, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
1.2.7.3
80
-
recombinant Kor, about 95% loss of activity under aerobic conditions, about 25% loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
1.2.7.3
80
-
recombinant For, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
1.2.7.3
90
-
recombinant For, about 90% loss of activity under aerobic conditions, about 30% loss of activity under anaerobic conditions
Hydrogenobacter thermophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.3
7.7
-
recombinant Kor
Hydrogenobacter thermophilus
1.2.7.3
8.7
-
recombinant For, at 70C, optimum varies between buffers
Hydrogenobacter thermophilus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.7.3
7
9.7
pH 7.0: about 45% of maximal activity, pH 9.7: about 65% of maximal activity, recombinant For
Hydrogenobacter thermophilus
Expression
EC Number
Organism
Commentary
Expression
1.2.7.3
Hydrogenobacter thermophilus
the For enzyme is expressed constitutively during oxygen respiration. Little expression of For is observed during cultivation under conditions of nitrate respiration; the Kor enzyme is constitutively expressed during respiration of either oxygen or nitrate respiration
additional information
General Information
EC Number
General Information
Commentary
Organism
1.2.7.3
physiological function
the For enzyme supports the optimal growth of the organism in the presence of oxygen; the For enzyme supports the optimal growth of the organism in the presence of oxygen
Hydrogenobacter thermophilus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.2.7.3
physiological function
the For enzyme supports the optimal growth of the organism in the presence of oxygen
Hydrogenobacter thermophilus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.2.7.3
Hydrogenobacter thermophilus
the Kor enzyme is constitutively expressed during respiration of either oxygen or nitrate respiration
additional information
1.2.7.3
Hydrogenobacter thermophilus
the For enzyme is expressed constitutively during oxygen respiration. Little expression of For is observed during cultivation under conditions of nitrate respiration
additional information