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Literature summary extracted from

  • Jeong, J.K.; Shin, H.J.; Kim, J.W.; Lee, C.H.; Kim, H.D.; Lim, W.K.
    Fluorescence and folding properties of Tyr mutant tryptophan synthase alpha-subunits from Escherichia coli (2003), Biochem. Biophys. Res. Commun., 300, 29-35.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 Y173F oligonucleotide-directed mutagenesis, gene trpA, alpha-subunit residue exchange, altered fluorescence and folding properties Escherichia coli
4.2.1.20 Y175F oligonucleotide-directed mutagenesis, gene trpA, alpha-subunit residue exchange, altered fluorescence and folding properties Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Escherichia coli
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.1.20 unfolding and refolding of wild-type and mutant alpha-subunits using urea, comparison of the folding process differences, thermodynamic parameters Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Escherichia coli D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Escherichia coli L-tryptophan + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 alphaTS
-
 Escherichia coli