BRENDA - Enzyme Database show

A novel five-subunit-type 2-oxoglutalate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6

Yun, N.R.; Yamamoto, M.; Arai, H.; Ishii, M.; Igarashi, Y.; Biochem. Biophys. Res. Commun. 292, 280-286 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.7.3
expression in Escherichia coli JM109
Hydrogenobacter thermophilus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus
involved in reductive TCA cycle
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
involved in reductive TCA cycle
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.3
Hydrogenobacter thermophilus
D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0
D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE
-
1.2.7.3
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0
D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.3
-
Hydrogenobacter thermophilus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.3
3.34
-
70°C, pH not specified in the publication, recombinant enzyme, oxidative decarboxylation of 2-oxoglutarate
Hydrogenobacter thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649592
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
involved in reductive TCA cycle
649592
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649592
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
involved in reductive TCA cycle
649592
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.3
More
five-subunit-structure: ForA, ForB, ForG, ForD, and ForE
Hydrogenobacter thermophilus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.3
70
-
assay at
Hydrogenobacter thermophilus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.3
expression in Escherichia coli JM109
Hydrogenobacter thermophilus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus
involved in reductive TCA cycle
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
involved in reductive TCA cycle
succinyl-CoA + CO2 + reduced ferredoxin
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.3
-
Hydrogenobacter thermophilus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.3
3.34
-
70°C, pH not specified in the publication, recombinant enzyme, oxidative decarboxylation of 2-oxoglutarate
Hydrogenobacter thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649592
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
involved in reductive TCA cycle
649592
Hydrogenobacter thermophilus
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
-
649592
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.3
2-oxoglutarate + CoA + oxidized ferredoxin
involved in reductive TCA cycle
649592
Hydrogenobacter thermophilus TK-6 / IAM 12695
succinyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.3
More
five-subunit-structure: ForA, ForB, ForG, ForD, and ForE
Hydrogenobacter thermophilus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.3
70
-
assay at
Hydrogenobacter thermophilus