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Literature summary extracted from

  • Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.; Ziegelhoffer, T.; Austin-Phillips, S.
    Cloned and expressed fungal phyA gene in alfalfa produces a stable phytase (2002), Biochem. Biophys. Res. Commun., 290, 1343-1348.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Medicago sativa leaves Aspergillus ficuum
3.1.3.26 expression in Medicago sativa leaves Aspergillus ficuum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 myo-inositol hexasulfate
-
 Aspergillus ficuum
3.1.3.8 Phenylglyoxal
-
 Aspergillus ficuum
3.1.3.26 myo-inositol hexasulfate
-
 Aspergillus ficuum
3.1.3.26 Phenylglyoxal
-
 Aspergillus ficuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.027
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, native enzyme Aspergillus ficuum
3.1.3.8 0.05
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, recombinant enzyme Aspergillus ficuum
3.1.3.26 0.027
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, native enzyme Aspergillus ficuum
3.1.3.26 0.05
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, recombinant enzyme Aspergillus ficuum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus ficuum
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Aspergillus ficuum
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein
-
 Aspergillus ficuum
3.1.3.26 glycoprotein
-
 Aspergillus ficuum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant enzyme Aspergillus ficuum
3.1.3.26 recombinant enzyme Aspergillus ficuum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 additional information
-
 692.0 nKat/ml, recombinant enzyme Aspergillus ficuum
3.1.3.26 additional information
-
 692.0 nKat/ml, recombinant enzyme Aspergillus ficuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus ficuum ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Aspergillus ficuum ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 More SDS-PAGE gives two broad bands in the regions 73000 Da and 100000 Da Aspergillus ficuum
3.1.3.26 More SDS-PAGE gives two broad bands in the regions 73000 Da and 100000 Da Aspergillus ficuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 58
-
 native and recombinant enzyme Aspergillus ficuum
3.1.3.26 58
-
 native and recombinant enzyme Aspergillus ficuum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 35 58 35°C: about 50% of maximal activity, 58°C: optimum, 68°C: enzyme is completely inactive, recombinant enzyme Aspergillus ficuum
3.1.3.26 35 58 35°C: about 50% of maximal activity, 58°C: optimum, 68°C: enzyme is completely inactive, recombinant enzyme Aspergillus ficuum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 300
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, recombinant enzyme Aspergillus ficuum
3.1.3.8 348
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, native enzyme Aspergillus ficuum
3.1.3.26 300
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, recombinant enzyme Aspergillus ficuum
3.1.3.26 348
-
 myo-inositol hexakisphosphate pH 5.0, 58°C, native enzyme Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
 recombinant enzyme Aspergillus ficuum
3.1.3.8 5 5.5 native enzyme Aspergillus ficuum
3.1.3.26 5
-
 recombinant enzyme Aspergillus ficuum
3.1.3.26 5 5.5 native enzyme Aspergillus ficuum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2.5 6 pH 2.5: about 35% of maximal activity, pH 6.0: about 25% of maximal activity, recombinant enzyme Aspergillus ficuum
3.1.3.26 2.5 6 pH 2.5: about 35% of maximal activity, pH 6.0: about 25% of maximal activity, recombinant enzyme Aspergillus ficuum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.8 0.00008
-
 myo-inositol hexasulfate pH 5.0, 58°C, recombinant enzyme Aspergillus ficuum
3.1.3.8 0.0046
-
 myo-inositol hexasulfate pH 5.0, 58°C, native enzyme Aspergillus ficuum
3.1.3.26 0.00008
-
 myo-inositol hexasulfate pH 5.0, 58°C, recombinant enzyme Aspergillus ficuum
3.1.3.26 0.0046
-
 myo-inositol hexasulfate pH 5.0, 58°C, native enzyme Aspergillus ficuum