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Literature summary extracted from

  • Njalsson, R.; Norgren, S.; Larsson, A.; Huang, C.S.; Anderson, M.E.; Luo, J.L.
    Cooperative binding of gamma-glutamyl substrate to human glutathione synthetase (2001), Biochem. Biophys. Res. Commun., 289, 80-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.3 expression in Escherichia coli BL21(DE3) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 additional information
-
 additional information kinetics of cooperative substrate binding Homo sapiens
6.3.2.3 0.063
-
 gamma-glutamyl-alpha-aminobutyrate pH 8.2, 37°C Homo sapiens
6.3.2.3 0.248
-
 ATP pH 8.2, 37°C Homo sapiens
6.3.2.3 0.452
-
 glycine pH 8.2, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly Homo sapiens final step of glutathione biosynthesis ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3 recombinant enzyme expressed in Escherichia coli, to homogeneity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-glutamyl-alpha-aminobutyrate
-
 Homo sapiens ?
-
 ?
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly gamma-glutamyl-cysteine is bound cooperatively by the enzyme, ATP might play a key role in sequential binding, but its amount does not alter the cooperative binding of gamma-glutamyl-cysteine Homo sapiens ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly final step of glutathione biosynthesis Homo sapiens ADP + phosphate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.3 Glutathione synthetase
-
 Homo sapiens
6.3.2.3 GS
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.3 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8.2
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.3 ATP
-
 Homo sapiens