EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | expression in Pichia pastoris | Aspergillus fumigatus |
3.1.3.8 | gene phyA is cloned and expressed in Pichia pastoris | Aspergillus fumigatus |
3.1.3.26 | expression in Pichia pastoris | Aspergillus fumigatus |
3.1.3.26 | gene phyA is cloned and expressed in Pichia pastoris | Aspergillus fumigatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | 1,2-Cyclohexanedione | 2 mM, 50% loss of activity | Aspergillus fumigatus | |
3.1.3.8 | myo-inositol hexasulfate | 0.01 mM, 50% inhibition | Aspergillus fumigatus | |
3.1.3.8 | Phenylglyoxal | - |
Aspergillus fumigatus | |
3.1.3.26 | 1,2,3-tricarboxycyclopentene-1 | 2 mM, 50% loss of activity | Aspergillus fumigatus | |
3.1.3.26 | 1,2-Cyclohexanedione | - |
Aspergillus fumigatus | |
3.1.3.26 | myo-inositol hexasulfate | 0.01 mM, 50% inhibition | Aspergillus fumigatus | |
3.1.3.26 | Phenylglyoxal | - |
Aspergillus fumigatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.03 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus fumigatus | |
3.1.3.8 | 0.04 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus ficuum | |
3.1.3.26 | 0.03 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus fumigatus | |
3.1.3.26 | 0.04 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus ficuum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 85000 | 100000 | - |
Aspergillus fumigatus |
3.1.3.8 | 85000 | 100000 | - |
Aspergillus ficuum |
3.1.3.26 | 85000 | 100000 | - |
Aspergillus fumigatus |
3.1.3.26 | 85000 | 100000 | - |
Aspergillus ficuum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Aspergillus ficuum | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
3.1.3.8 | Aspergillus fumigatus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
3.1.3.26 | Aspergillus ficuum | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
3.1.3.26 | Aspergillus fumigatus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | recombinant enzyme | Aspergillus fumigatus |
3.1.3.26 | recombinant enzyme | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | 4-nitrophenyl phosphate + H2O | - |
Aspergillus fumigatus | 4-nitrophenol + phosphate | - |
? | |
3.1.3.8 | 4-nitrophenyl phosphate + H2O | - |
Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | ? + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | ? + phosphate | - |
? | |
3.1.3.26 | 4-nitrophenyl phosphate + H2O | - |
Aspergillus fumigatus | 4-nitrophenol + phosphate | - |
? | |
3.1.3.26 | 4-nitrophenyl phosphate + H2O | - |
Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | ? + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | ? + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | More | a broad proptein band of 85000-100000 Da is observed after SDS-PAGE | Aspergillus fumigatus |
3.1.3.8 | More | a broad protein band of 85000-100000 Da is observed after SDS-PAGE | Aspergillus ficuum |
3.1.3.26 | More | a broad proptein band of 85000-100000 Da is observed after SDS-PAGE | Aspergillus fumigatus |
3.1.3.26 | More | a broad protein band of 85000-100000 Da is observed after SDS-PAGE | Aspergillus ficuum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 58 | - |
- |
Aspergillus fumigatus |
3.1.3.8 | 58 | - |
- |
Aspergillus ficuum |
3.1.3.26 | 58 | - |
- |
Aspergillus fumigatus |
3.1.3.26 | 58 | - |
- |
Aspergillus ficuum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 20 | 75 | 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity | Aspergillus fumigatus |
3.1.3.8 | 20 | 75 | 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity, reaction with phytate | Aspergillus fumigatus |
3.1.3.26 | 20 | 75 | 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity | Aspergillus fumigatus |
3.1.3.26 | 20 | 75 | 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity, reaction with phytate | Aspergillus fumigatus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 22 | - |
2 min, stable | Aspergillus fumigatus |
3.1.3.8 | 70 | - |
2 min, 42% loss of activity | Aspergillus fumigatus |
3.1.3.8 | 70 | - |
2 min, 42% loss of activity, irreversible | Aspergillus fumigatus |
3.1.3.8 | 80 | - |
2 min, 92% loss of activity | Aspergillus fumigatus |
3.1.3.8 | 80 | - |
2 min, 92% loss of activity, irreversible | Aspergillus fumigatus |
3.1.3.26 | 22 | - |
2 min, stable | Aspergillus fumigatus |
3.1.3.26 | 70 | - |
2 min, 42% loss of activity | Aspergillus fumigatus |
3.1.3.26 | 70 | - |
2 min, 42% loss of activity, irreversible | Aspergillus fumigatus |
3.1.3.26 | 80 | - |
2 min, 92% loss of activity | Aspergillus fumigatus |
3.1.3.26 | 80 | - |
2 min, 92% loss of activity, irreversible | Aspergillus fumigatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 46 | - |
myo-inositol hexakisphosphate | - |
Aspergillus fumigatus | |
3.1.3.8 | 46 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus fumigatus | |
3.1.3.8 | 348 | - |
myo-inositol hexakisphosphate | - |
Aspergillus ficuum | |
3.1.3.8 | 348 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus ficuum | |
3.1.3.26 | 46 | - |
myo-inositol hexakisphosphate | - |
Aspergillus fumigatus | |
3.1.3.26 | 46 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus fumigatus | |
3.1.3.26 | 348 | - |
myo-inositol hexakisphosphate | - |
Aspergillus ficuum | |
3.1.3.26 | 348 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C | Aspergillus ficuum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 4 | - |
4-nitrophenyl phosphate as substrate | Aspergillus fumigatus |
3.1.3.8 | 4 | - |
reaction with 4-nitrophenyl phosphate | Aspergillus fumigatus |
3.1.3.8 | 5 | - |
- |
Aspergillus ficuum |
3.1.3.8 | 5 | - |
and a second optimum at pH 2.5 | Aspergillus ficuum |
3.1.3.8 | 5 | - |
myo-inositol hexakisphosphate as substrate | Aspergillus fumigatus |
3.1.3.8 | 5 | - |
reaction with phytate | Aspergillus fumigatus |
3.1.3.26 | 4 | - |
4-nitrophenyl phosphate as substrate | Aspergillus fumigatus |
3.1.3.26 | 4 | - |
reaction with 4-nitrophenyl phosphate | Aspergillus fumigatus |
3.1.3.26 | 5 | - |
- |
Aspergillus ficuum |
3.1.3.26 | 5 | - |
and a second optimum at pH 2.5 | Aspergillus ficuum |
3.1.3.26 | 5 | - |
myo-inositol hexakisphosphate as substrate | Aspergillus fumigatus |
3.1.3.26 | 5 | - |
reaction with phytate | Aspergillus fumigatus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 4 | 6 | pH 3.0: no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, 4-nitrophenyl phosphate as substrate | Aspergillus fumigatus |
3.1.3.8 | 4 | 6 | pH 4.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, myo-inositol hexakisphosphate as substrate | Aspergillus fumigatus |
3.1.3.8 | 4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 50% of maximal activity, reaction with phytate | Aspergillus fumigatus |
3.1.3.8 | 4 | 5 | pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate | Aspergillus fumigatus |
3.1.3.26 | 4 | 6 | pH 3.0: no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, 4-nitrophenyl phosphate as substrate | Aspergillus fumigatus |
3.1.3.26 | 4 | 6 | pH 4.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, myo-inositol hexakisphosphate as substrate | Aspergillus fumigatus |
3.1.3.26 | 4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 50% of maximal activity, reaction with phytate | Aspergillus fumigatus |
3.1.3.26 | 4 | 5 | pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate | Aspergillus fumigatus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.00064 | - |
myo-inositol hexasulfate | ph 5.0, 58°C | Aspergillus fumigatus | |
3.1.3.26 | 0.00064 | - |
myo-inositol hexasulfate | ph 5.0, 58°C | Aspergillus fumigatus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.3.8 | Aspergillus ficuum | - |
- |
4.94 |
3.1.3.8 | Aspergillus fumigatus | - |
- |
7.04 |
3.1.3.26 | Aspergillus ficuum | - |
- |
4.94 |
3.1.3.26 | Aspergillus fumigatus | - |
- |
7.04 |