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Literature summary extracted from

  • Ullah, A.H.; Sethumadhavan, K.; Lei, X.G.; Mullaney, E.J.
    Biochemical characterization of cloned Aspergillus fumigatus phytase (phyA) (2000), Biochem. Biophys. Res. Commun., 275, 279-285.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Pichia pastoris Aspergillus fumigatus
3.1.3.8 gene phyA is cloned and expressed in Pichia pastoris Aspergillus fumigatus
3.1.3.26 expression in Pichia pastoris Aspergillus fumigatus
3.1.3.26 gene phyA is cloned and expressed in Pichia pastoris Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 1,2-Cyclohexanedione 2 mM, 50% loss of activity Aspergillus fumigatus
3.1.3.8 myo-inositol hexasulfate 0.01 mM, 50% inhibition Aspergillus fumigatus
3.1.3.8 Phenylglyoxal
-
Aspergillus fumigatus
3.1.3.26 1,2,3-tricarboxycyclopentene-1 2 mM, 50% loss of activity Aspergillus fumigatus
3.1.3.26 1,2-Cyclohexanedione
-
Aspergillus fumigatus
3.1.3.26 myo-inositol hexasulfate 0.01 mM, 50% inhibition Aspergillus fumigatus
3.1.3.26 Phenylglyoxal
-
Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.03
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus fumigatus
3.1.3.8 0.04
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus ficuum
3.1.3.26 0.03
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus fumigatus
3.1.3.26 0.04
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus ficuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 85000 100000
-
Aspergillus fumigatus
3.1.3.8 85000 100000
-
Aspergillus ficuum
3.1.3.26 85000 100000
-
Aspergillus fumigatus
3.1.3.26 85000 100000
-
Aspergillus ficuum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus ficuum
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Aspergillus fumigatus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Aspergillus ficuum
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Aspergillus fumigatus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant enzyme Aspergillus fumigatus
3.1.3.26 recombinant enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 4-nitrophenyl phosphate + H2O
-
Aspergillus fumigatus 4-nitrophenol + phosphate
-
?
3.1.3.8 4-nitrophenyl phosphate + H2O
-
Aspergillus ficuum 4-nitrophenol + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus ficuum myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus ? + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus ficuum ? + phosphate
-
?
3.1.3.26 4-nitrophenyl phosphate + H2O
-
Aspergillus fumigatus 4-nitrophenol + phosphate
-
?
3.1.3.26 4-nitrophenyl phosphate + H2O
-
Aspergillus ficuum 4-nitrophenol + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Aspergillus ficuum myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus ? + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Aspergillus ficuum ? + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 More a broad proptein band of 85000-100000 Da is observed after SDS-PAGE Aspergillus fumigatus
3.1.3.8 More a broad protein band of 85000-100000 Da is observed after SDS-PAGE Aspergillus ficuum
3.1.3.26 More a broad proptein band of 85000-100000 Da is observed after SDS-PAGE Aspergillus fumigatus
3.1.3.26 More a broad protein band of 85000-100000 Da is observed after SDS-PAGE Aspergillus ficuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 58
-
-
Aspergillus fumigatus
3.1.3.8 58
-
-
Aspergillus ficuum
3.1.3.26 58
-
-
Aspergillus fumigatus
3.1.3.26 58
-
-
Aspergillus ficuum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 20 75 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity Aspergillus fumigatus
3.1.3.8 20 75 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity, reaction with phytate Aspergillus fumigatus
3.1.3.26 20 75 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity Aspergillus fumigatus
3.1.3.26 20 75 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity, reaction with phytate Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 22
-
2 min, stable Aspergillus fumigatus
3.1.3.8 70
-
2 min, 42% loss of activity Aspergillus fumigatus
3.1.3.8 70
-
2 min, 42% loss of activity, irreversible Aspergillus fumigatus
3.1.3.8 80
-
2 min, 92% loss of activity Aspergillus fumigatus
3.1.3.8 80
-
2 min, 92% loss of activity, irreversible Aspergillus fumigatus
3.1.3.26 22
-
2 min, stable Aspergillus fumigatus
3.1.3.26 70
-
2 min, 42% loss of activity Aspergillus fumigatus
3.1.3.26 70
-
2 min, 42% loss of activity, irreversible Aspergillus fumigatus
3.1.3.26 80
-
2 min, 92% loss of activity Aspergillus fumigatus
3.1.3.26 80
-
2 min, 92% loss of activity, irreversible Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 46
-
myo-inositol hexakisphosphate
-
Aspergillus fumigatus
3.1.3.8 46
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus fumigatus
3.1.3.8 348
-
myo-inositol hexakisphosphate
-
Aspergillus ficuum
3.1.3.8 348
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus ficuum
3.1.3.26 46
-
myo-inositol hexakisphosphate
-
Aspergillus fumigatus
3.1.3.26 46
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus fumigatus
3.1.3.26 348
-
myo-inositol hexakisphosphate
-
Aspergillus ficuum
3.1.3.26 348
-
myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4
-
4-nitrophenyl phosphate as substrate Aspergillus fumigatus
3.1.3.8 4
-
reaction with 4-nitrophenyl phosphate Aspergillus fumigatus
3.1.3.8 5
-
-
Aspergillus ficuum
3.1.3.8 5
-
and a second optimum at pH 2.5 Aspergillus ficuum
3.1.3.8 5
-
myo-inositol hexakisphosphate as substrate Aspergillus fumigatus
3.1.3.8 5
-
reaction with phytate Aspergillus fumigatus
3.1.3.26 4
-
4-nitrophenyl phosphate as substrate Aspergillus fumigatus
3.1.3.26 4
-
reaction with 4-nitrophenyl phosphate Aspergillus fumigatus
3.1.3.26 5
-
-
Aspergillus ficuum
3.1.3.26 5
-
and a second optimum at pH 2.5 Aspergillus ficuum
3.1.3.26 5
-
myo-inositol hexakisphosphate as substrate Aspergillus fumigatus
3.1.3.26 5
-
reaction with phytate Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 4 6 pH 3.0: no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, 4-nitrophenyl phosphate as substrate Aspergillus fumigatus
3.1.3.8 4 6 pH 4.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, myo-inositol hexakisphosphate as substrate Aspergillus fumigatus
3.1.3.8 4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 50% of maximal activity, reaction with phytate Aspergillus fumigatus
3.1.3.8 4 5 pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate Aspergillus fumigatus
3.1.3.26 4 6 pH 3.0: no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, 4-nitrophenyl phosphate as substrate Aspergillus fumigatus
3.1.3.26 4 6 pH 4.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, myo-inositol hexakisphosphate as substrate Aspergillus fumigatus
3.1.3.26 4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 50% of maximal activity, reaction with phytate Aspergillus fumigatus
3.1.3.26 4 5 pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate Aspergillus fumigatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.8 0.00064
-
myo-inositol hexasulfate ph 5.0, 58°C Aspergillus fumigatus
3.1.3.26 0.00064
-
myo-inositol hexasulfate ph 5.0, 58°C Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Aspergillus ficuum
-
-
4.94
3.1.3.8 Aspergillus fumigatus
-
-
7.04
3.1.3.26 Aspergillus ficuum
-
-
4.94
3.1.3.26 Aspergillus fumigatus
-
-
7.04