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Literature summary extracted from

  • Moon, T.C.; Kim, M.S.; Lee, S.J.; Lee, T.Y.; Kwon, S.H.; Baek, S.H.; Chang, H.W.
    Detection and characterization of 45 kDa platelet activating factor acetylhydrolase in cerebrospinal fluid of children with meningitis (2003), Arch. Pharm. Res., 26, 554-558.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.47 dithiothreitol 1.3fold increase in activity, isozyme II from cerebrospinal fluid Homo sapiens
3.1.1.47 additional information 3fold increase in activity in subjects with meningitis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.47 iodoacetamide 17% inhibition at 5 mM Homo sapiens
3.1.1.47 additional information no inhibition of the isozyme II from cerebrospinal fluid by NaF, Mg2+, Ca2+, and EDTA Homo sapiens
3.1.1.47 p-bromophenacylbromide 60% inhibition at 5 mM, isozyme II from cerebrospinal fluid Homo sapiens
3.1.1.47 PMSF 80% inhibition at 5 mM, complete inhibition at 10 mM, isozyme II from cerebrospinal fluid Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.47 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.47 additional information enzyme from cerebrospinal fluid is Ca2+-independent Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.47 45000
-
 1 * 45000, isozyme II, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O Homo sapiens i.e. platelet-activation factor 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 additional information Homo sapiens isozyme II activity is 3fold increased insubjects suffering meningitis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Homo sapiens
-
 children with meningitis
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 cerebrospinal fluid isozyme II, of children with meningitis Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 1-acyl-2-linoleoyl-glycero-phosphocholine + H2O
-
 Homo sapiens 2-linoleoyl-glycero-phosphocholine + acylic acid
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 additional information isozyme II from cerebrospinal fluid does not degrade phospholipids with a long chain fatty acyl group at sn-2 position Homo sapiens ?
-
 ?
3.1.1.47 additional information isozyme II activity is 3fold increased insubjects suffering meningitis Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 monomer 1 * 45000, isozyme II, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.47 PAF-AH
-
 Homo sapiens
3.1.1.47 platelet-activating factor acetylhydrolase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.47 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.47 40
-
 30 min, stable up to, gradually decrease at temperatures above 40°C Homo sapiens
3.1.1.47 70
-
 30 min, inactivation Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.47 7.4
-
 assay at Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.47 4 11 broad range, inactivation below pH 4.0 Homo sapiens