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Literature summary extracted from

  • Rudolph, B.; Hansen, T.; Schonheit, P.
    Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily (2004), Arch. Microbiol., 181, 82-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.9 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.9 6-phosphogluconate competitive Methanocaldococcus jannaschii
5.3.1.9 erythrose 4-phosphate competitive Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.9 0.04
-
 D-fructose 6-phosphate 50°C, pH 6.3 Methanocaldococcus jannaschii
5.3.1.9 1
-
 D-glucose 6-phosphate 50°C, pH 6.3 Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.9 45000
-
 2 * 45000, SDS-PAGE Methanocaldococcus jannaschii
5.3.1.9 80000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.9 Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.9 D-glucose 6-phosphate
-
 Methanocaldococcus jannaschii D-fructose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.9 dimer 2 * 45000, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.9 89
-
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.9 60 95 60°C: about 30% of maximal activity, 95°C: about 30% of maximal activity Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.9 80
-
 120 min, stable Methanocaldococcus jannaschii
5.3.1.9 90
-
 120 min, about 40% loss of activity Methanocaldococcus jannaschii
5.3.1.9 95
-
 120 min, about 90% loss of activity Methanocaldococcus jannaschii
5.3.1.9 100
-
 3 min, complete loss of activity Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.9 6.3
-
-
 Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.9 5.3 7 50% of maximal activity at pH 6.3 and pH 7.0 Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.9 0.02
-
 6-phosphogluconate 50°C, pH 6.3 Methanocaldococcus jannaschii
5.3.1.9 0.06
-
 erythrose 4-phosphate 50°C, pH 6.3 Methanocaldococcus jannaschii