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Literature summary extracted from

  • Rittmann, D.; Schaffer, S.; Wendisch, V.F.; Sahm, H.
    Fructose-1,6-bisphosphatase from Corynebacterium glutamicum: expression and deletion of the fbp gene and biochemical characterization of the enzyme (2003), Arch. Microbiol., 180, 285-292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 xpression with a N-terminal His-tag in Escherichia coli Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP-Mn
-
 Corynebacterium glutamicum
3.1.3.11 D-fructose-1-phosphate
-
 Corynebacterium glutamicum
3.1.3.11 D-fructose-2,6-bisphosphate
-
 Corynebacterium glutamicum
3.1.3.11 Li+
-
 Corynebacterium glutamicum
3.1.3.11 MnAMP
-
 Corynebacterium glutamicum
3.1.3.11 MnUDP
-
 Corynebacterium glutamicum
3.1.3.11 phosphate
-
 Corynebacterium glutamicum
3.1.3.11 phosphoenolpyruvate
-
 Corynebacterium glutamicum
3.1.3.11 UDP-Mn
-
 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.014
-
 D-fructose-1,6-bisphosphate pH 7.7, 30°C Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+
-
 Corynebacterium glutamicum
3.1.3.11 Mn2+
-
 Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 35500
-
 4 * 35500, SDS-PAGE Corynebacterium glutamicum
3.1.3.11 140000
-
-
 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Corynebacterium glutamicum enzyme is usually regarded as a regulatory enzyme of gluconeogenesis D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 the recombinant enzyme Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 additional information
-
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Corynebacterium glutamicum D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O enzyme is usually regarded as a regulatory enzyme of gluconeogenesis Corynebacterium glutamicum D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-glucose 6-phosphate + H2O 23% of the activity found with D-fructose 1,6-bisphosphate Corynebacterium glutamicum D-glucose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 homotetramer 4 * 35500, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 FBPase II
-
 Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 3.2
-
 D-fructose-1,6-bisphosphate pH 7.7, 30°C Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.11 0.09
-
 AMP-Mn pH 7.7, 30°C Corynebacterium glutamicum
3.1.3.11 0.14
-
 Li+ pH 7.7, 30°C Corynebacterium glutamicum
3.1.3.11 0.36
-
 phosphoenolpyruvate pH 7.7, 30°C Corynebacterium glutamicum
3.1.3.11 1.2
-
 D-fructose-1-phosphate pH 7.7, 30°C Corynebacterium glutamicum
3.1.3.11 1.6
-
 phosphate pH 7.7, 30°C Corynebacterium glutamicum
3.1.3.11 3.4
-
 D-fructose-2,6-bisphosphate pH 7.7, 30°C Corynebacterium glutamicum
3.1.3.11 5
-
 UDP-Mn pH 7.7, 30°C Corynebacterium glutamicum