EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | ADP | - |
Methanocaldococcus jannaschii | |
6.4.1.1 | ATP | - |
Methanocaldococcus jannaschii | |
6.4.1.1 | Avidin | 100 molar excess amount completely inhibits after 5 min | Methanocaldococcus jannaschii | |
6.4.1.1 | KCl | activates at lower concentrations, inhibits at higher concentrations | Methanocaldococcus jannaschii | |
6.4.1.1 | Mg2+ | when Mg2+ concentration in the assay exceeds that of ATP | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.1 | 0.22 | - |
HCO3- | 80°C, pH 8.5 | Methanocaldococcus jannaschii | |
6.4.1.1 | 0.374 | - |
ATP | 80°C, pH 8.5 | Methanocaldococcus jannaschii | |
6.4.1.1 | 0.53 | - |
pyruvate | 80°C, pH 8.5 | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | Co2+ | can poorly replace Mg2+ | Methanocaldococcus jannaschii | |
6.4.1.1 | KCl | optimal concentration: 0.175 mM, activates at lower concentrations, inhibits at higher concentrations | Methanocaldococcus jannaschii | |
6.4.1.1 | Mg2+ | required, inhibits at higher concentrations | Methanocaldococcus jannaschii | |
6.4.1.1 | Mn2+ | can poorly replace Mg2+ | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.1 | 55000 | - |
alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE | Methanocaldococcus jannaschii |
6.4.1.1 | 65000 | - |
alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE | Methanocaldococcus jannaschii |
6.4.1.1 | 480000 | - |
gel filtration, Stokes' radius | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.1 | ATP + pyruvate + HCO3- | Methanocaldococcus jannaschii | - |
ADP + phosphate + oxaloacetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.1 | Methanocaldococcus jannaschii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.1 | - |
Methanocaldococcus jannaschii |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.1 | -20°C, stable, purified enzyme, 75% activity after 5 days | Methanocaldococcus jannaschii |
6.4.1.1 | -80°C, stable, purified enzyme, 75% activity after 5 days | Methanocaldococcus jannaschii |
6.4.1.1 | 4°C, stable, purified enzyme, 83-85% activity after 36 h | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.1 | ATP + pyruvate + HCO3- | - |
Methanocaldococcus jannaschii | ADP + phosphate + oxaloacetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.1 | octamer | alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.1 | 80 | 90 | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.1 | 80 | - |
rapid inactivation, t1/2: 1 h in 100 mM Tris/HCl, pH 7, sulfate salts provide partial protection | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.1 | 8.5 | - |
- |
Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | biotin | - |
Methanocaldococcus jannaschii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.1 | 0.89 | - |
ADP | 80°C, pH 8.5 | Methanocaldococcus jannaschii | |
6.4.1.1 | 5.34 | - |
ATP | 80°C, pH 8.5 | Methanocaldococcus jannaschii |