BRENDA - Enzyme Database

Kinetic studies and site-directed mutagenesis of Escherichia coli agmatinase. A role for Glu274 in binding and correct positioning of the substrate guanidinium group

Carvajal, N.; Orellana, M.S.; Salas, M.; Enriquez, P.; Alarcon, R.; Uribe, E.; Lopez, V.; Arch. Biochem. Biophys. 430, 185-190 (2004)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.11
E274A
1-2% of wild type activity
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
guanidinium ion
competitive
Escherichia coli
3.5.3.11
putrescine
competitive
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
1.1
-
agmatine
wild type
Escherichia coli
3.5.3.11
6.3
-
agmatine
mutant E274A
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.11
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
649495
Escherichia coli
putrescine + urea
-
649495
Escherichia coli
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.11
15
-
guanidinium ion
wild type
Escherichia coli
3.5.3.11
44.2
-
guanidinium ion
mutant E274A
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.11
E274A
1-2% of wild type activity
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
guanidinium ion
competitive
Escherichia coli
3.5.3.11
putrescine
competitive
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.11
15
-
guanidinium ion
wild type
Escherichia coli
3.5.3.11
44.2
-
guanidinium ion
mutant E274A
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
1.1
-
agmatine
wild type
Escherichia coli
3.5.3.11
6.3
-
agmatine
mutant E274A
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
649495
Escherichia coli
putrescine + urea
-
649495
Escherichia coli
?