EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.16 | - |
Abies grandis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.16 | additional information | exchange of the predicted helix D through F region gives rise to an (-)-limonene/(-)-alpha-pinene synthase product outcome | Abies grandis |
4.2.3.119 | additional information | to elucidate critical amino acids involved in determining monoterpene product distribution, a combination of domain swapping and reciprocal site-directed mutagenesis was carried out between (-)-(4S)-limonene synthase LS and (-)-(4S)-limonene/(-)-(1S, 5S)-alpha-pinene synthase LPS. Amino acids in the predicted D through F helix regions are critical for product determination. Chimera consisting of N-terminal 218 residues of LS plus corresponding C-terminus of LPS produces 20.7% alpha-pinene, 11.2% sabinene, 7.1% beta-pinene, 25.6% limonene, 35.4% beta-phellandrene, with 42.8% relative activity. Chimera consisting of N-terminal 518 residues of LS plus corresponding C-terminus of LPS produces 6.4% alpha-pinene, 1.5% sabinene, 11% beta-pinene, 64.1% limonene, 17% beta-phellandrene, with 101% relative activity. Chimera consisting of N-terminal 442 residues of LPS plus corresponding C-terminus of LS produces 11% alpha-pinene, 1.4% sabinene, 6.3% beta-pinene, 47.9% limonene, 33.3% beta-phellandrene, with 41.7% relative activity | Abies grandis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.16 | Abies grandis | - |
grand fir | - |
4.2.3.119 | Abies grandis | Q9M7C9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.16 | geranyl diphosphate | - |
Abies grandis | (-)-(4S)-limonene + diphosphate | - |
? | |
4.2.3.119 | geranyl diphosphate | - |
Abies grandis | (-)-alpha-pinene + diphosphate | products are 20% (-)-alpha-pinene, 6% (-)-beta-pinene, 26% (-)-limonene, 37% (-)-9-beta-phellandrene, and about 11% other monoterpenes | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.119 | (-)-limonene/(-)-alpha-pinene synthase | - |
Abies grandis |
4.2.3.119 | LPS | - |
Abies grandis |