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Literature summary extracted from
- The temperature dependence of activity and structure for the most prevalent mutant aldolase B associated with hereditary fructose intolerance (2002), Arch. Biochem. Biophys., 408, 295-304.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.2.13 | medicine | mutations in aldolase B gene cause hereditary fructose intolerance, most common intolerance allele encodes a A149P substitution | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.13 | enzyme from patients with fructose intolerance | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.13 | 0.0017 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens |  |
| 4.1.2.13 | 0.0024 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
| 4.1.2.13 | 0.0028 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 0.0085 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 0.24 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
| 4.1.2.13 | 0.88 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
| 4.1.2.13 | 4.2 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 16.4 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 57000 | - |
sucrose density gradient centrifugation, 30°C | Homo sapiens |
| 4.1.2.13 | 71000 | - |
sucrose density gradient centrifugation, 4°C | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.13 | D-fructose 1,6-bisphosphate | Homo sapiens | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.13 | Homo sapiens | - |
patients with fructose intolerance | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.13 | enzyme from patients with fructose intolerance | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.13 | D-fructose 1,6-bisphosphate | - |
Homo sapiens | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| 4.1.2.13 | D-Fructose 1-phosphate | - |
Homo sapiens | Glycerone phosphate + D-glyceraldehyde | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.13 | dimer | gel filtration, enzyme from patients with fructose intolerance | Homo sapiens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 15 | - |
gradual loss of activity above, enzyme from patients with fructose intolerance | Homo sapiens |
| 4.1.2.13 | 40 | - |
wild-type enzyme, activity is stable up to 40°C, declines sharply above 40°C | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 10 | - |
enzyme from patients with fructose intolerance shows increased temperature sensitivity, activity at 10°C is 6fold lower than that of the wild-type, and activity decreases substantially at higher temperatures | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.13 | 0.008 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 0.011 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 0.019 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 0.029 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
| 4.1.2.13 | 0.12 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
| 4.1.2.13 | 0.19 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
| 4.1.2.13 | 2 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
| 4.1.2.13 | 2.1 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
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