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Literature summary extracted from

  • MacDonald, J.A.; Storey, K.B.
    Purification and characterization of fructose bisphosphate aldolase from the ground squirrel, Spermophilus lateralis: enzyme role in mammalian hibernation (2002), Arch. Biochem. Biophys., 408, 279-285.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 (NH4)2SO4 50% inhibition at 2.1 mM at 37°C, 50% inhibition at 1.9 mM at 5°C Callospermophilus lateralis
4.1.2.13 (NH4)2SO4 50% inhibition at 6.2 mM at 37°C, 50% inhibition at 5.27 mM at 5°C Oryctolagus cuniculus
4.1.2.13 ADP 50% inhibition at 1.6 mM at 37°C, 50% inhibition at 1.7 mM at 5°C Callospermophilus lateralis
4.1.2.13 ADP 50% inhibition at 2.7 mM at 37°C, 50% inhibition at 5.4 mM at 5°C Oryctolagus cuniculus
4.1.2.13 AMP 50% inhibition at 2.3 mM at 37°C, 50% inhibition at 2.6 mM at 5°C Callospermophilus lateralis
4.1.2.13 AMP 50% inhibition at 4.5 mM at 37°C, 50% inhibition at 6.0 mM at 5°C Oryctolagus cuniculus
4.1.2.13 ATP 50% inhibition at 1.4 mM at 37°C, 50% inhibition at 1.1 mM at 5°C Callospermophilus lateralis
4.1.2.13 ATP 50% inhibition at 2.1 mM at 37°C, 50% inhibition at 2.1 mM at 5°C Oryctolagus cuniculus
4.1.2.13 citrate 50% inhibition at 2.4 mM at 37°C, 50% inhibition at 1.9 mM at 5°C Callospermophilus lateralis
4.1.2.13 citrate 50% inhibition at 2.7 mM at 37°C, 50% inhibition at 3.4 mM at 5°C Oryctolagus cuniculus
4.1.2.13 IMP 50% inhibition at 1.3 mM at 37°C, 50% inhibition at 1.3 mM at 5°C Callospermophilus lateralis
4.1.2.13 IMP 50% inhibition at 4.2 mM at 37°C, 50% inhibition at 8.5 mM at 5°C Oryctolagus cuniculus
4.1.2.13 KCl 50% inhibition at 38.2 mM at 37°C, 50% inhibition at 36.1 mM at 5°C Callospermophilus lateralis
4.1.2.13 KCl 50% inhibition at 111 mM at 37°C, 50% inhibition at 63.3 mM at 5°C Oryctolagus cuniculus
4.1.2.13 Magnesium citrate 50% inhibition at 1.9 mM at 37°C, 50% inhibition at 2.5 mM at 5°C Callospermophilus lateralis
4.1.2.13 Magnesium citrate 50% inhibition at 9 mM at 37°C, 50% inhibition at 2.5 mM at 5°C Oryctolagus cuniculus
4.1.2.13 NaCl 50% inhibition at 14.7 mM at 37°C, 50% inhibition at 31 mM at 5°C Callospermophilus lateralis
4.1.2.13 NaCl 50% inhibition at 110 mM at 37°C, 50% inhibition at 52 mM at 5°C Oryctolagus cuniculus
4.1.2.13 NH4Cl 50% inhibition at 51.5 mM at 37°C, 50% inhibition at 34.1 mM at 5°C Callospermophilus lateralis
4.1.2.13 NH4Cl 50% inhibition at 166 mM at 37°C, 50% inhibition at 40.6 mM at 5°C Oryctolagus cuniculus
4.1.2.13 phosphate 50% inhibition at 1.7 mM at 37°C, 50% inhibition at 0.63 mM at 5°C Callospermophilus lateralis
4.1.2.13 phosphate 50% inhibition at 6.1 mM at 37°C, 50% inhibition at 1.7 mM at 5°C Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0009
-
 D-fructose 1,6-bisphosphate pH 7.3, 37°C Callospermophilus lateralis
4.1.2.13 0.0013
-
 D-fructose 1,6-bisphosphate pH 7.3, 37°C Oryctolagus cuniculus
4.1.2.13 0.00145
-
 D-fructose 1,6-bisphosphate pH 7.3, 5°C Callospermophilus lateralis
4.1.2.13 0.00411
-
 D-fructose 1,6-bisphosphate pH 7.3, 5°C Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 40000
-
 4 * 40000, SDS-PAGE Callospermophilus lateralis
4.1.2.13 159000
-
 gel filtration Callospermophilus lateralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate Oryctolagus cuniculus
-
 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate Callospermophilus lateralis
-
 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Callospermophilus lateralis
-
 golden-mantled ground squirrel
-
4.1.2.13 Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13 homogeneity Callospermophilus lateralis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.13 muscle
-
 Callospermophilus lateralis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Oryctolagus cuniculus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Callospermophilus lateralis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.13 tetramer 4 * 40000, SDS-PAGE Callospermophilus lateralis

Synonyms

EC Number Synonyms Comment Organism
4.1.2.13 F1,6P2 aldolase
-
 Callospermophilus lateralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 6.3 7.5
-
 Callospermophilus lateralis
4.1.2.13 7
-
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.13 7.5
-
 steep drop in activity above Callospermophilus lateralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.13 0.00023
-
 phosphate pH 7.3, 5°C Callospermophilus lateralis
4.1.2.13 0.00118
-
 phosphate pH 7.3, 37°C Callospermophilus lateralis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.13 Callospermophilus lateralis isoelectric focusing, pH gradient pH 3.5 - pH 10
-
 5.5