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Literature summary extracted from

  • Salas, J.J.; Ohlrogge, J.B.
    Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases (2002), Arch. Biochem. Biophys., 403, 25-34.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 overexpression of wild-type isozymes and chimeric mutants in Escherichia coli Bl21(DE3), the chimeric mutants are constructed by combination of the N-terminus of FatA with the C-terminus of FatB resulting in FatAB, or vice versa resulting in FatBA Coriandrum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.14 additional information
-
additional information Km is slightly influenced by substrate chain length and double bonds Arabidopsis thaliana
3.1.2.14 additional information
-
additional information Km is slightly influenced by substrate chain length and double bonds Coriandrum sativum
3.1.2.14 0.0014
-
oleoyl-[acyl-carrier protein] pH 8.0, 22°C Spinacia oleracea
3.1.2.14 0.0026
-
oleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0026
-
palmitoleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0029
-
myristoleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.003
-
stearoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0031
-
oleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0038
-
palmitoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.004
-
myristoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0049
-
palmitoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0049
-
palmitoleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.005
-
stearoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0089
-
myristoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.0139
-
myristoleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.14 plastid stroma soluble Spinacia oleracea 9532
-
3.1.2.14 plastid stroma nuclear gene encoded, targeted to the plastids, soluble Arabidopsis thaliana 9532
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O Spinacia oleracea
-
oleic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O Arabidopsis thaliana
-
oleic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O Coriandrum sativum
-
oleic acid + acyl-carrier protein
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Arabidopsis thaliana
-
isozyme FatA and FatB
-
3.1.2.14 Coriandrum sativum
-
isozyme FatA
-
3.1.2.14 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 partial purification Spinacia oleracea
3.1.2.14 partial purification of the isozymes and the recombinant chimeric mutants Coriandrum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.14 leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 additional information substrate specificities of isozymes and chimeric mutants, kcat is strongly influenced by substrate chain length and double bonds Coriandrum sativum ?
-
?
3.1.2.14 additional information the substrate specificity determinants are located at the N-terminus of the isozymes, kcat is strongly influenced by substrate chain length and double bonds Arabidopsis thaliana ?
-
?
3.1.2.14 myristoleoyl-[acyl-carrier protein] + H2O
-
Coriandrum sativum myristoleic acid + acyl-carrier protein
-
?
3.1.2.14 myristoleoyl-[acyl-carrier protein] + H2O low activity Arabidopsis thaliana myristoleic acid + acyl-carrier protein
-
?
3.1.2.14 myristoyl-[acyl-carrier protein] + H2O low activity Arabidopsis thaliana myristic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O
-
Spinacia oleracea oleic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O
-
Arabidopsis thaliana oleic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O
-
Coriandrum sativum oleic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O preferred substrate of recombinant chimeric mutant FatAB Arabidopsis thaliana oleic acid + acyl-carrier protein
-
?
3.1.2.14 palmitoleoyl-[acyl-carrier protein] + H2O
-
Arabidopsis thaliana palmitoleic acid + acyl-carrier protein
-
?
3.1.2.14 palmitoleoyl-[acyl-carrier protein] + H2O
-
Coriandrum sativum palmitoleic acid + acyl-carrier protein
-
?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O
-
Coriandrum sativum palmitic acid + acyl-carrier protein
-
?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O preferred substrate of recombinant chimeric mutant FatBA, isozyme FatB prefers [acyl-carrier-protein]-substrate of spinach before the Escherichia coli derived Arabidopsis thaliana palmitic acid + acyl-carrier protein
-
?
3.1.2.14 stearoyl-[acyl-carrier protein] + H2O
-
Arabidopsis thaliana stearic acid + acyl-carrier protein
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 12:0-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 12:0-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 12:0-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 12:0-acyl-carrier protein thioesterase
-
Spinacia oleracea
3.1.2.14 12:0-acyl-carrier protein thioesterase
-
Arabidopsis thaliana
3.1.2.14 12:0-acyl-carrier protein thioesterase
-
Coriandrum sativum
3.1.2.14 14:0-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 14:0-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 14:0-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 14:0-acyl-carrier protein thioesterase
-
Spinacia oleracea
3.1.2.14 14:0-acyl-carrier protein thioesterase
-
Arabidopsis thaliana
3.1.2.14 14:0-acyl-carrier protein thioesterase
-
Coriandrum sativum
3.1.2.14 16:0-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 16:0-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 16:0-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 16:0-acyl-carrier protein thioesterase
-
Spinacia oleracea
3.1.2.14 16:0-acyl-carrier protein thioesterase
-
Arabidopsis thaliana
3.1.2.14 16:0-acyl-carrier protein thioesterase
-
Coriandrum sativum
3.1.2.14 18:0-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 18:0-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 18:0-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 18:0-acyl-carrier protein thioesterase
-
Spinacia oleracea
3.1.2.14 18:0-acyl-carrier protein thioesterase
-
Arabidopsis thaliana
3.1.2.14 18:0-acyl-carrier protein thioesterase
-
Coriandrum sativum
3.1.2.14 acyl-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 acyl-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 acyl-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 acyl-ACP-hydrolase
-
Spinacia oleracea
3.1.2.14 acyl-ACP-hydrolase
-
Arabidopsis thaliana
3.1.2.14 acyl-ACP-hydrolase
-
Coriandrum sativum
3.1.2.14 acyl-acyl carrier protein hydrolase
-
Spinacia oleracea
3.1.2.14 acyl-acyl carrier protein hydrolase
-
Arabidopsis thaliana
3.1.2.14 acyl-acyl carrier protein hydrolase
-
Coriandrum sativum
3.1.2.14 acyl-acyl carrier protein thioesterase
-
Spinacia oleracea
3.1.2.14 acyl-acyl carrier protein thioesterase
-
Arabidopsis thaliana
3.1.2.14 acyl-acyl carrier protein thioesterase
-
Coriandrum sativum
3.1.2.14 acyl-[acyl-carrier-protein] hydrolase
-
Spinacia oleracea
3.1.2.14 acyl-[acyl-carrier-protein] hydrolase
-
Arabidopsis thaliana
3.1.2.14 acyl-[acyl-carrier-protein] hydrolase
-
Coriandrum sativum
3.1.2.14 AH
-
Spinacia oleracea
3.1.2.14 AH
-
Arabidopsis thaliana
3.1.2.14 AH
-
Coriandrum sativum
3.1.2.14 BTE
-
Spinacia oleracea
3.1.2.14 BTE
-
Arabidopsis thaliana
3.1.2.14 BTE
-
Coriandrum sativum
3.1.2.14 C12:0 ACP TE
-
Spinacia oleracea
3.1.2.14 C12:0 ACP TE
-
Arabidopsis thaliana
3.1.2.14 C12:0 ACP TE
-
Coriandrum sativum
3.1.2.14 C18:1-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 C18:1-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 C18:1-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 Deubiquitinating enzyme 14
-
Spinacia oleracea
3.1.2.14 Deubiquitinating enzyme 14
-
Arabidopsis thaliana
3.1.2.14 Deubiquitinating enzyme 14
-
Coriandrum sativum
3.1.2.14 hydrolase, oleoyl-[acyl carrier protein]
-
Spinacia oleracea
3.1.2.14 hydrolase, oleoyl-[acyl carrier protein]
-
Arabidopsis thaliana
3.1.2.14 hydrolase, oleoyl-[acyl carrier protein]
-
Coriandrum sativum
3.1.2.14 oleoyl-ACP thioesterase
-
Spinacia oleracea
3.1.2.14 oleoyl-ACP thioesterase
-
Arabidopsis thaliana
3.1.2.14 oleoyl-ACP thioesterase
-
Coriandrum sativum
3.1.2.14 oleoyl-acyl carrier protein thioesterase
-
Spinacia oleracea
3.1.2.14 oleoyl-acyl carrier protein thioesterase
-
Arabidopsis thaliana
3.1.2.14 oleoyl-acyl carrier protein thioesterase
-
Coriandrum sativum
3.1.2.14 OTE
-
Spinacia oleracea
3.1.2.14 OTE
-
Arabidopsis thaliana
3.1.2.14 OTE
-
Coriandrum sativum
3.1.2.14 PATE
-
Spinacia oleracea
3.1.2.14 PATE
-
Arabidopsis thaliana
3.1.2.14 PATE
-
Coriandrum sativum
3.1.2.14 STE
-
Spinacia oleracea
3.1.2.14 STE
-
Arabidopsis thaliana
3.1.2.14 STE
-
Coriandrum sativum
3.1.2.14 Ubiquitin thiolesterase 14
-
Spinacia oleracea
3.1.2.14 Ubiquitin thiolesterase 14
-
Arabidopsis thaliana
3.1.2.14 Ubiquitin thiolesterase 14
-
Coriandrum sativum
3.1.2.14 Ubiquitin-specific processing protease 14
-
Spinacia oleracea
3.1.2.14 Ubiquitin-specific processing protease 14
-
Arabidopsis thaliana
3.1.2.14 Ubiquitin-specific processing protease 14
-
Coriandrum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.14 22
-
room temperature, assay at Spinacia oleracea
3.1.2.14 22
-
room temperature, assay at Coriandrum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.14 additional information
-
additional information recombinant chimeric mutants FatAB and FatBA Arabidopsis thaliana
3.1.2.14 0.004
-
myristoleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.008
-
myristoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.02
-
myristoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.04
-
palmitoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.12
-
palmitoleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.15
-
stearoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.22
-
myristoleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.74
-
palmitoleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 0.91
-
stearoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 1.23
-
oleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 1.94
-
oleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 2.4
-
palmitoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 2.94
-
oleoyl-[acyl-carrier protein] isozyme FatA, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 6.08
-
stearoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 6.08
-
oleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana
3.1.2.14 6.08
-
palmitoleoyl-[acyl-carrier protein] isozyme FatB, pH 8.0, 22°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.14 8
-
assay at Spinacia oleracea
3.1.2.14 8
-
assay at Coriandrum sativum