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Literature summary extracted from

  • Vogel, A.; Borchers, T.; Marcus, K.; Meyer, H.E.; Krebs, B.; Spener, F.
    Heterologous expression and characterization of recombinant purple acid phosphatase from red kidney bean (2002), Arch. Biochem. Biophys., 401, 164-172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expression in High Five insect cells Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 H2O2 native and recombinant enzyme Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe2+ native enzyme with an iron-zinc center is not activated upon reduction of the enzyme with ferrous ions and ascorbate, but the activity of recombinant enzyme is increased about 4fold Phaseolus vulgaris
3.1.3.2 Iron native enzyme with an iron-zinc center is not activated upon reduction of the enzyme with ferrous ions and ascorbate, but the activity of recombinant enzyme is increased about 4fold Phaseolus vulgaris
3.1.3.2 Zinc native enzyme with an iron-zinc center is not activated upon reduction of the enzyme with ferrous ions and ascorbate, but the activity of recombinant enzyme is increased about 4fold Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Phaseolus vulgaris O24319
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein the enzyme contains five N-linked glycosylation sites at Asn81, Asn109, Asn143, Asn211 and Asn396 Phaseolus vulgaris

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 recombinant enzyme Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 265.8
-
-
 Phaseolus vulgaris

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 kbPAP
-
 Phaseolus vulgaris
3.1.3.2 purple acid phosphatase
-
 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 6.1
-
 native and recombinant enzyme Phaseolus vulgaris