BRENDA - Enzyme Database

Cloning and characterization of an S-formylglutathione hydrolase from Arabidopsis thaliana

Kordic, S.; Cummins, I.; Edwards, R.; Arch. Biochem. Biophys. 399, 232-238 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.2.12
additional information
not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.12
DNA and amino acid sequence determinationand analysis, expression in Escherichia coli as C-terminally His-tagged enzyme
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
Cu2+
90% reduced activity at 0.01 mM
Arabidopsis thaliana
3.1.2.12
additional information
no inhibition by PMSF, not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
3.1.2.12
N-ethylmaleimide
complete inhibition at 1 mM
Arabidopsis thaliana
3.1.2.12
Zn2+
50% reduced activity at 0.01 mM
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.03
-
fluorescein diacetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.12
-
4-yethylumbelliferyl acetate
recombinant enzyme, pH 7.5, 37°C
Arabidopsis thaliana
3.1.2.12
0.13
-
S-formylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.15
-
S-acetylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.54
-
beta-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.57
-
Alpha-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
1.02
-
4-nitrophenyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
31700
-
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
3.1.2.12
66000
-
recombinant enzyme, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Arabidopsis thaliana
enzyme is involved in formaldehyde detoxification
glutathione + formate
-
Arabidopsis thaliana
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.12
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.12
cell suspension culture
-
Arabidopsis thaliana
-
3.1.2.12
plant
-
Arabidopsis thaliana
-
3.1.2.12
root
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.12
0.08
-
recombinant enzyme, substrate fluorescein diacetate
Arabidopsis thaliana
3.1.2.12
1.3
-
recombinant enzyme, substrate beta-naphthyl acetate
Arabidopsis thaliana
3.1.2.12
5.7
-
recombinant enzyme, substrate 4-nitrophenyl acetate
Arabidopsis thaliana
3.1.2.12
10.4
-
recombinant enzyme, substrate S-formylglutathione
Arabidopsis thaliana
3.1.2.12
14.5
-
recombinant enzyme, substrate S-acetylglutathione
Arabidopsis thaliana
3.1.2.12
15.2
-
recombinant enzyme, substrate alpha-naphthyl acetate
Arabidopsis thaliana
3.1.2.12
36.3
-
recombinant enzyme, substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-methylumbelliferyl acetate + H2O
-
649455
Arabidopsis thaliana
4-methylumbelliferol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
4-nitrophenyl acetate + H2O
-
649455
Arabidopsis thaliana
4-nitrophenol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
alpha-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
alpha-naphthol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
beta-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
beta-naphthol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
fluorescein diacetate + H2O
-
649455
Arabidopsis thaliana
?
-
649455
Arabidopsis thaliana
?
3.1.2.12
S-acetylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
enzyme is involved in formaldehyde detoxification
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
37
-
assay at
Arabidopsis thaliana
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.12
55
-
fully stable up to, rapid inactivation at higher temperatures
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
7.2
-
assay at, except with substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
3.1.2.12
7.6
8
optimum
Arabidopsis thaliana
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.2.12
Arabidopsis thaliana
isoelectric focusing
-
6
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.2.12
additional information
not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
DNA and amino acid sequence determinationand analysis, expression in Escherichia coli as C-terminally His-tagged enzyme
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
Cu2+
90% reduced activity at 0.01 mM
Arabidopsis thaliana
3.1.2.12
additional information
no inhibition by PMSF, not affected by Ca2+, Mn2+, Mg2+, and EDTA, not affected by methanol, phenobarbital and dichlormid
Arabidopsis thaliana
3.1.2.12
N-ethylmaleimide
complete inhibition at 1 mM
Arabidopsis thaliana
3.1.2.12
Zn2+
50% reduced activity at 0.01 mM
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.03
-
fluorescein diacetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.12
-
4-yethylumbelliferyl acetate
recombinant enzyme, pH 7.5, 37°C
Arabidopsis thaliana
3.1.2.12
0.13
-
S-formylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.15
-
S-acetylglutathione
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.54
-
beta-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
0.57
-
Alpha-naphthyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
3.1.2.12
1.02
-
4-nitrophenyl acetate
recombinant enzyme, pH 7.2, 37°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
31700
-
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
3.1.2.12
66000
-
recombinant enzyme, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Arabidopsis thaliana
enzyme is involved in formaldehyde detoxification
glutathione + formate
-
Arabidopsis thaliana
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.12
cell suspension culture
-
Arabidopsis thaliana
-
3.1.2.12
plant
-
Arabidopsis thaliana
-
3.1.2.12
root
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.12
0.08
-
recombinant enzyme, substrate fluorescein diacetate
Arabidopsis thaliana
3.1.2.12
1.3
-
recombinant enzyme, substrate beta-naphthyl acetate
Arabidopsis thaliana
3.1.2.12
5.7
-
recombinant enzyme, substrate 4-nitrophenyl acetate
Arabidopsis thaliana
3.1.2.12
10.4
-
recombinant enzyme, substrate S-formylglutathione
Arabidopsis thaliana
3.1.2.12
14.5
-
recombinant enzyme, substrate S-acetylglutathione
Arabidopsis thaliana
3.1.2.12
15.2
-
recombinant enzyme, substrate alpha-naphthyl acetate
Arabidopsis thaliana
3.1.2.12
36.3
-
recombinant enzyme, substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-methylumbelliferyl acetate + H2O
-
649455
Arabidopsis thaliana
4-methylumbelliferol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
4-nitrophenyl acetate + H2O
-
649455
Arabidopsis thaliana
4-nitrophenol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
alpha-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
alpha-naphthol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
beta-naphthyl acetate + H2O
-
649455
Arabidopsis thaliana
beta-naphthol + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
fluorescein diacetate + H2O
-
649455
Arabidopsis thaliana
?
-
649455
Arabidopsis thaliana
?
3.1.2.12
S-acetylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + acetate
-
649455
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
-
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
3.1.2.12
S-formylglutathione + H2O
enzyme is involved in formaldehyde detoxification
649455
Arabidopsis thaliana
glutathione + formate
-
649455
Arabidopsis thaliana
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 31700, recombinant enzyme, amino acid sequence calculation without His-tag
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
37
-
assay at
Arabidopsis thaliana
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.12
55
-
fully stable up to, rapid inactivation at higher temperatures
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
7.2
-
assay at, except with substrate 4-methylumbelliferyl acetate
Arabidopsis thaliana
3.1.2.12
7.6
8
optimum
Arabidopsis thaliana
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.2.12
Arabidopsis thaliana
isoelectric focusing
-
6